Changes in serum and exudate creatine phosphokinase concentrations as an indicator of deep tissue injury: a pilot study
Changes in serum and exudate creatine phosphokinase concentrations as an indicator of deep tissue injury: a pilot study
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DOI：
10.1111/j.1742-481x.2008.00543.x
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发表时间：
2008-12-01
影响因子：
3.1
通讯作者：
Sugama, Junko
中科院分区：
文献类型：
--
作者：
Sari, Yunita;Nakagami, Gojiro;Sugama, Junko
深部组织损伤(DTI)早期难以发现。肌酸磷酸激酶(CPK)作为一种肌肉酶可作为DTI的一个有前景的指标。然而，血清CPK水平反映的是全身状况，而不是局部伤口环境。伤口渗出物可以作为局部伤口环境的指标。本研究旨在探讨伤口渗出液中CPK水平作为DTI指标的有效性。将大鼠分为对照组、6h 10 kg组和6h 20 kg组。伤前、伤后8、12h、1、2、3d采血，第2、3d取渗出液，10、20 kg组在伤后8、12h血清CPK水平较基础值和对照组显著升高，第1天降至正常水平，两组伤后第2天渗出液CPK水平升高，第3天下降。这是第一项表明伤口渗出物中CPK作为DTI指标的可能性的研究。
Deep tissue injury (DTI) is difficult to detect in the early phase. Creatine phosphokinase (CPK) as a muscle enzyme could represent a promising indicator of DTI. However, serum CPK levels reflect the systemic condition rather than the local wound environment. Wound exudates can be indicative of the local wound environment. This study aimed to investigate the usefulness of CPK levels in wound exudates as an indicator of DTI. Rats were divided into control, 6 hours 10-kg and 6 hours 20-kg loading groups. Serum samples were obtained before wounding, and at 8 and 12 hours, and 1, 2 and 3 days after wounding, while exudate samples were obtained on days 2 and 3. Serum CPK levels were markedly increased in the 10-kg and 20-kg groups at 8 and 12 hours after loading compared with the baseline value and control group, but decreased to the normal level on day 1. In both loading groups, exudate CPK levels were high on day 2 and decreased on day 3. Muscle necrosis was more severe in the 20-kg group than in the 10-kg group by histological examination. This is the first study to indicate the potential of CPK in wound exudates as an indicator of DTI.