Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification
Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification
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DOI：
10.1016/j.talanta.2016.07.018
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发表时间：
2017-03-01
期刊：
影响因子：
6.1
通讯作者：
Kong, Jilie
中科院分区：
文献类型：
--
作者：
Cao, Hongmei;Fang, Xueen;Kong, Jilie
粘蛋白1（MUC-1）是一种在结肠直肠癌、肺癌、前列腺癌、胰腺癌、卵巢癌和膀胱癌等恶性上皮细胞表面过表达的糖蛋白，已被证实是诊断早期癌症的有用生物标志物。因此，建立敏感、特异的MUC-1检测方法对肿瘤的早期诊断具有重要意义。本文报道了一种新的基于适体的免疫环介导等温扩增（Im-LAMP）技术，利用对MUC-1具有高亲和力的MUC-1适体定量检测MUC-1。循环时间线性依赖于粘蛋白1浓度的对数（log），范围为1.0 pM至1.0 aM。我们将这种技术称为Im-LAMP，这是一种用于检测低丰度蛋白质的新方法，具有高灵敏度（通过计算，检测限为120个MUC-1分子）和特异性。最后，该方法也成功地应用于人血清样品的分析。这也为不同类型低丰度癌症生物标志物的早期诊断奠定了基础。
Mucin 1 (MUC-1), a glycoprotein over-expressed on most malignant epithelial cell surfaces, such as colorectal, lung, prostate, pancreatic, ovarian, and bladder carcinomas, has been confirmed as a useful biomarker for the diagnosis of early cancers. Therefore, it is very important for early cancer diagnoses to develop the sensitive and specific detection approach of MUC-1. In this paper, we report a novel technique of aptamer-based Immuno-Loop-Mediated Isothermal Amplification (Im-LAMP) for the quantitative detection of MUC-1 by MUC-1 aptamer with high affinity for MUC-1. The cycle time is linearly dependent on the logarithm (log) of the concentration of mucin 1 ranging from 1.0 pM to 1.0 aM. We call this technique Im-LAMP, which is a novel method for the detection of low-abundance proteins with high sensitivity (a low detection limit of 120 MUC-1 molecules by calculation)and specificity. Finally, this approach is also successfully applied in the analysis of human blood serum samples. It also lays the foundation for the early diagnosis of different types of low abundance cancer biomarkers.