Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days of culture
Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days of culture
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DOI：
10.1016/s0015-0282(99)00458-6
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发表时间：
2000-01-01
影响因子：
6.7
通讯作者：
Pieters, MHEC
中科院分区：
文献类型：
--
作者：
Huisman, GJ;Fauser, BCJM;Pieters, MHEC
目的：评估移植最多两个培养3-5天的胚胎后接受IVF的患者的植入率和妊娠率。设计：前瞻性研究。设置：三级转诊大学医院的IVF实验室。患者：1787对夫妇，他们在1995年1月至1997年12月期间接受了第一个IVF周期。干预：体外受精和胚胎移植后3，4，或5天的培养，使用单一的培养基没有coculture.Main结果Measures（S）：种植和妊娠率。结果（S）：总体种植和妊娠率没有显着不同的培养期。所有可用胚胎的41%在第5天发育成囊胚。62%的患者可以移植至少一个优质囊胚。囊胚的植入率为26%，而8细胞胚胎在第3天的植入率为18%。植入率为延迟，正常，和先进的胚胎没有显着不同的延长culture periods. Conclusions（S）：在研究条件下，移植后5天，而不是3天的胚胎培养的胚胎没有改变的整体植入率和妊娠率。可用于移植的胚胎的植入潜力可以在延长的培养期后更好地评估。五天的培养可以减少胚胎数量，而不会降低整体妊娠率。《生育与无菌》2000;73：117-22。（C）1999年由美国生殖医学学会。
Objective: To evaluate implantation and pregnancy rates in patients undergoing IVF after the transfer of a maximum of two embryos that had been cultured for 3-5 days.Design: Prospective study.Setting: An IVF laboratory at a tertiary referral university hospital.Patient(s): One thousand seven hundred eighty-seven couples who underwent their first IVF cycle between January 1995 and December 1997.Intervention(s): In vitro fertilization and transfer of embryos after 3, 4, or 5 days of culture using a single medium without coculture.Main Outcome Measure(s): Implantation and pregnancy rates.Result(s): Overall implantation and pregnancy rates were not significantly different with different culture periods. Forty-one percent of all available embryos developed into blastocysts on day 5. The transfer of at least one good-quality blastocyst could be performed in 62% of patients. Blastocysts had an implantation rate of 26% per embryo, whereas the implantation rate of eight-cell embryos on day 3 was 18%. Implantation rates for retarded, normal, and advanced embryos were not significantly different with an extended culture period.Conclusion(s): Under the study conditions, the transfer of embryos after 5 days rather than 3 days of embryo culture did not change the overall implantation and pregnancy rates. The implantation potential of embryos available for transfer can be assessed better after an extended culture period. Five days of culture allows the transfer of a reduced number of embryos without decreasing overall pregnancy rates. Fertil Steril(R) 2000;73: 117-22. (C)1999 by American Society for Reproductive Medicine.