Retention and mass transfer characteristics in reversed-phase liquid chromatography using a tetrahydrofuran-water solution as the mobile phase.
Retention and mass transfer characteristics in reversed-phase liquid chromatography using a tetrahydrofuran-water solution as the mobile phase.
复制标题
使用四氢呋喃-水溶液作为流动相的反相液相色谱中的保留和传质特性。
DOI：
10.1016/s0021-9673(01)00821-4
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
G. Guiochon
中科院分区：
文献类型：
--
作者：
K. Miyabe;S. Sotoura;G. Guiochon
在C18-硅胶-四氢呋喃-水（50：50，v/v）体系中测定了反相液相色谱（RPLC）的保留特性和传质动力学。这些参数分别来自洗脱峰的一阶矩和二阶矩。进一步的信息，该系统的热力学性质来自这些时刻的温度依赖性。一些先前建立的相关性，证实了该系统，即，保留和表面扩散和线性自由能关系的熵补偿。这些结果与在使用甲醇-水（70：30，v/v）和乙腈-水（70：30，v/v）溶液的其它类似系统中观察到的结果进行了比较。C18-硅胶颗粒中的表面扩散对颗粒内扩散的贡献被证明是重要的。对表面扩散热力学性质的分析表明，在这三种反相液相色谱体系中，表面扩散的活化能均小于吸附热。移动的相组成对描述保留和传质动力学的参数的影响的性质和程度是不同的，但RPLC系统中涉及的色谱机理似乎相似，与移动的相中有机改性剂的性质无关。
The characteristics of the retention and the mass transfer kinetics in reversed-phase liquid chromatography (RPLC) were measured on a system consisting of a C18-silica gel and a tetrahydrofuran–water (50:50, v/v) solution. These parameters were derived from the first and the second moments of the elution peaks, respectively. Further information on the thermodynamic properties of this system was derived from the temperature dependence of these moments. Some correlations previously established were confirmed for this system, namely, an enthalpy–entropy compensation for both retention and surface diffusion and a linear free-energy relationship. These results are compared with those observed in other similar systems using methanol–water (70:30, v/v) and acetonitrile–water (70:30, v/v) solutions. The contribution of surface diffusion to intraparticle diffusion in C18-silica gel particles was shown to be important. The analysis of the thermodynamic properties of surface diffusion suggests that, in these three RPLC systems, its activation energy is lower than the isosteric heat of adsorption. The nature and the extent of the influence of the mobile phase composition on the parameters describing the retention and the mass transfer kinetics are different but the chromatographic mechanisms involved in RPLC systems appear similar, irrespective of the nature of the organic modifier in the mobile phase.