Nuclear posttranscriptional processing of thymidine kinase mRNA at the onset of DNA synthesis.
Nuclear posttranscriptional processing of thymidine kinase mRNA at the onset of DNA synthesis.
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DNA 合成开始时胸苷激酶 mRNA 的核转录后加工。
DOI：
10.1073/pnas.85.13.4705
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发表时间：
1988
影响因子：
11.1
通讯作者：
Pardee,AB
中科院分区：
文献类型：
--
作者：
Gudas,JM;Knight,GB;Pardee,AB
在 BALB/c 3T3 细胞从细胞周期的 G0 期进展到 S 期期间，研究了胸苷激酶 (TK) mRNA 积累的转录后调节机制。在 G0 或 G1 期收获的细胞的细胞核或细胞质区室中只能检测到很少的 TK mRNA。然而，在 S 期开始时，核 TK mRNA 前体和成熟 TK mRNA 的水平急剧增加。在S期细胞的细胞核中检测到的高分子量TK异质核RNA种类被聚腺苷酸化并与源自TK基因的内含子序列杂交。在放线菌素 D 存在的情况下，一系列高分子量前体可以被追逐成较低分子量的物质，这表明内含子序列的有序去除与前体-产物关系的动力学有关。这些结果表明，TK 异质核 RNA 在 G1-S 边界的核转录后加工发生了显着变化，此外，还定义了用于检查体内 RNA 加工事件的模型系统。
The posttranscriptional regulatory mechanism(s) underlying thymidine kinase (TK) mRNA accumulation was investigated in BALB/c 3T3 cells during their progression from G0 into S phase of the cell cycle. Very little TK mRNA could be detected in either the nuclear or the cytoplasmic compartment from cells harvested in G0 or G1. At the onset of S phase, however, the level of nuclear TK mRNA precursors and mature TK mRNAs increased dramatically. The high molecular weight TK heterogeneous nuclear RNA species detected in the nuclei of S-phase cells were polyadenylylated and hybridized to intron sequences derived from the TK gene. A series of high molecular weight precursors could be chased to lower molecular weight species in the presence of actinomycin D, suggesting an ordered removal of intron sequences with the kinetics of a precursor-product relationship. These results demonstrate a striking change in the nuclear posttranscriptional processing of TK heterogeneous nuclear RNA at the G1-S boundary and, furthermore, define a model system for the examination of RNA-processing events in vivo.