Efficient method for visualization and isolation of proteins resolved in polyacrylamide gels.
Efficient method for visualization and isolation of proteins resolved in polyacrylamide gels.
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DOI：
10.1016/s0021-9673(01)92699-8
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
R. T. Francis;J. Davie;M. Sayre;E. Rocha;F. Ziemer;G. Riedel
中科院分区：
文献类型：
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作者：
R. T. Francis;J. Davie;M. Sayre;E. Rocha;F. Ziemer;G. Riedel
聚丙烯酰胺凝胶电泳是一种常用的纯化蛋白质的方法，用于重组实验、氨基酸组成和序列测定。在本报告中，我们描述了将在蛋白质的分离和表征中普遍使用的方法，以及在电泳托盘缓冲液中用硼酸代替甘氨酸的好处。我们还描述了如何用8-苯胺-1-萘磺酸快速可视化各种凝胶体系（包括含有十二烷基硫酸钠的凝胶体系）中溶解的蛋白质，并有效地转移到第二个凝胶进行二维凝胶分析，或通过电洗脱分离用于后续表征。
Polyacrylamide gel electrophoresis is a popular method used to purify proteins for reconstitution experiments, amino acid composition and sequence determination. In this report we describe methods that will be of general use in the isolation and characterization of proteins and the benefits of substituting boric acid for glycine in the electrophoresis tray buffers. We also described how proteins resolved in a variety of gel systems (including those containing sodium dodecyl sulfate) may be rapidly visuallized with 8-anilino-1-naphthalene sulfonic acid and efficiently transferred to a second gel for two-dimensional gel analysis, or isolated by electroelution for subsequent characterization.