Chromosome-level genome assembly for the angiosperm Silene conica.
Chromosome-level genome assembly for the angiosperm Silene conica.
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被子植物 Silene conica 的染色体水平基因组组装。
DOI：
10.1101/2023.09.05.556365
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Sloan,DanielB
中科院分区：
文献类型：
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作者：
Fields,PeterD;Weber,MelodyM;Waneka,Gus;Broz,AmandaK;Sloan,DanielB
被子植物silene属是生态学和进化领域广泛研究的对象，但高质量的参考基因组序列的可用性有限。在这里，我们报道了一个基于太平洋生物科学HiFi， Hi-C和Bionano技术的染色体水平的基因组组装。该组合产生了10个支架（每条染色体1个），总长度为862 Mb，间隙含量仅为~ 1%。这些结果证实了先前的观察。与该属祖先的12个染色体相比，conica及其近亲的碱基染色体数目减少了。独角蝽有一个特别大的线粒体基因组（bbbb11 Mb），主要由未知来源的序列组成。对共享序列内容的分析表明，核DNA的转移不太可能是线粒体基因组扩增的主要驱动因素。从更广泛的意义上说，这一组合将为未来的基因组研究提供宝贵的资源，包括与最近进化出性染色体的相关物种的比较分析。
The angiosperm genusSilenehas been the subject of extensive study in the field of ecology and evolution, but the availability of high-quality reference genome sequences has been limited for this group. Here, we report a chromosome-level assembly for the genome ofSilene conicabased on Pacific Bioscience HiFi, Hi-C, and Bionano technologies. The assembly produced 10 scaffolds (1 per chromosome) with a total length of 862 Mb and only ∼1% gap content. These results confirm previous observations thatS. conicaand its relatives have a reduced base chromosome number relative to the genus's ancestral state of 12.Silene conicahas an exceptionally large mitochondrial genome (>11 Mb), predominantly consisting of sequence of unknown origins. Analysis of shared sequence content suggests that it is unlikely that transfer of nuclear DNA is the primary driver of this mitochondrial genome expansion. More generally, this assembly should provide a valuable resource for future genomic studies inSilene, including comparative analyses with related species that recently evolved sex chromosomes.