Aurora-A breaks symmetry in contractile actomyosin networks independently of its role in centrosome maturation
Aurora-A breaks symmetry in contractile actomyosin networks independently of its role in centrosome maturation
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Aurora-A 打破收缩肌动球蛋白网络的对称性，与其在中心体成熟中的作用无关
DOI：
10.1016/j.devcel.2019.02.012
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发表时间：
2019
影响因子：
11.8
通讯作者：
F. Motegi
中科院分区：
文献类型：
--
作者：
P. Zhao;X. Teng;S. N. Tantirimudalige;M. Nishikawa;T. Wohland;Y. Toyama;F. Motegi
在细胞皮层肌动蛋白骨架的重排促进了细胞的极性。触发收缩肌动球蛋白网络不对称重塑的程序仍然知之甚少。在这里，我们发现秀丽隐杆线虫受精卵的极化是通过有丝分裂激酶Aurora-A对皮质肌动球蛋白网络的顺序下调而建立的。极光- a在中心体周围积聚，局部破坏近端皮层的肌动球蛋白收缩活动，从而促进对称破缺时皮层的流动。Aurora-A随后介导皮质肌动球蛋白网络的全局分解，通过抑制中心体非依赖性皮质血流促进初始极化。Aurora-A从细胞质到皮质的易位足以干扰皮质肌动球蛋白网络，而不依赖于其在中心体成熟和细胞周期进程中的作用。我们认为，Aurora-A活性作为中心体介导的线索，打破了肌动球蛋白收缩活动的对称性，并通过抑制皮质肌动球蛋白网络促进了初始极化。
Cell polarity is facilitated by a rearrangement of the actin cytoskeleton at the cell cortex. The program triggering the asymmetric remodeling of contractile actomyosin networks remains poorly understood. Here, we show that polarization ofCaenorhabditis eleganszygotes is established through sequential downregulation of cortical actomyosin networks by the mitotic kinase, Aurora-A. Aurora-A accumulates around centrosomes to locally disrupt the actomyosin contractile activity at the proximal cortex, thereby promoting cortical flows during symmetry breaking. Aurora-A later mediates global disassembly of cortical actomyosin networks, which facilitates the initial polarization through suppression of centrosome-independent cortical flows. Translocation of Aurora-A from the cytoplasm to the cortex is sufficient to interfere with the cortical actomyosin networks independently of its roles in centrosome maturation and cell-cycle progression. We propose that Aurora-A activity serves as a centrosome-mediated cue that breaks symmetry in actomyosin contractile activity, and facilitates the initial polarization through global suppression of cortical actomyosin networks.