Expression of macrophage-derived chemokine (MDC)/CCL22 in human lung cancer
Expression of macrophage-derived chemokine (MDC)/CCL22 in human lung cancer
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DOI：
10.1007/s00262-006-0133-y
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发表时间：
2006-11-01
影响因子：
5.8
通讯作者：
Shimokata, Kaoru
中科院分区：
文献类型：
--
作者：
Nakanishi, Toru;Imaizumi, Kazuyoshi;Shimokata, Kaoru
背景资料：CXCR 3趋化因子的配体[10 kD的IFN-γ诱导蛋白（IP-10/CXCL 10），IFN-γ诱导的单核因子（Mig/CXCL 9），IFN-诱导的T细胞a化学引诱物（I-TAC/CXCL 11）]和CCR 4 [巨噬细胞源性趋化因子（MDC/CCL22），胸腺和活化调节的趋化因子（TARC/CCL 17）]已显示在T辅助细胞募集到组织中中起中心作用。为了研究这些趋化因子在肺癌肿瘤进展中的作用，我们研究了它们在人肺癌组织中的表达，以确定它们的表达与患者预后之间的可能关系。方法：从40例肺癌组织（腺癌24例，鳞癌16例）中提取总RNA。我们通过实时定量RT-PCR测量趋化因子（IP-10、Mig、I-TAC、MDC和TARC）的基因表达水平。结果如下：肺癌组织中MDC基因的高表达与较长的无病生存期和较低的肿瘤切除后复发风险显著相关。我们没有发现IP-10、Mig、I-TAC和TARC基因表达与无病生存或手术后复发风险降低有任何显著关系。结论：结果提示，肿瘤组织中MDC基因表达增高可能是改善肺癌预后的一个预测指标。
Background: Ligands for CXCR3 chemokines [IFN-gamma-inducible protein of 10 kD (IP-10/CXCL10), monokine induced by IFN-gamma (Mig/CXCL9), IFN-inducible T cell a chemoattractant (I-TAC/CXCL11)] and those for CCR4 [macrophage-derived chemokine (MDC/CCL22), thymus- and activation-regulated chemokine (TARC/CCL17)] have been shown to play the central roles for T helper-cell recruitment into the tissues. To examine the role of these chemokines in tumor progression of lung cancer, we investigated their expression in human lung cancer tissues to determine the possible relationship between their expression and the prognosis of patients. Methods: Total RNA was prepared from lung cancer tissues of 40 patients ( 24 adenocarcinoma and 16 squamous cell carcinoma). We measured gene expression levels of chemokines (IP-10, Mig, I-TAC, MDC and TARC) by real-time quantitative RT-PCR. Results: Higher gene expression of MDC in lung cancer was significantly correlated with longer disease-free survival time and lower risk of recurrence after tumor resection. We could not find any significant relationship of IP-10, Mig, I-TAC and TARC gene expression with disease-free survival or lower risk of recurrence after surgery. Conclusions: These results suggest that increased gene expression of MDC in tumor tissues may be a predictive marker for improving the prognosis of lung cancer.