A subset of notch functions during Drosophila eye development require Su(H) and the E(spl) gene complex.
A subset of notch functions during Drosophila eye development require Su(H) and the E(spl) gene complex.
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发表时间：
1998-08
期刊：
影响因子：
4.6
通讯作者：
P. Ligoxygakis;Sung-Yun Yu;C. Delidakis;N. Baker
中科院分区：
文献类型：
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作者：
P. Ligoxygakis;Sung-Yun Yu;C. Delidakis;N. Baker
Notch信号通路参与许多决定细胞命运的过程。先前的工作表明，在果蝇眼睛的 R8 规范过程中，连续步骤都需要 Notch。最初，Notch 增强无调性表达并促进无调性功能。无调性自动调节建立后，Notch 信号传导会抑制横向规范期间的无调性表达。在本文中，我们研究了 Notch 通路的哪些已知成分参与了每个信号传导过程。使用克隆分析，我们表明，Notch 的配体 Delta 与 Notch 一起是原神经增强和横向规范所必需的，而下游组件 Suppressor-of-Hairless 和 Enhancer-of-Split 仅涉及横向规范。我们的数据表明，Notch 在原神经增强过程中存在独特的信号转导途径。通过错误表达实验，我们还表明特定的分裂增强子 bHLH 基因对横向规范的贡献可能有很大差异。
The Notch signalling pathway is involved in many processes where cell fate is decided. Previous work showed that Notch is required at successive steps during R8 specification in the Drosophila eye. Initially, Notch enhances atonal expression and promotes atonal function. After atonal autoregulation has been established, Notch signalling represses atonal expression during lateral specification. In this paper we investigate which known components of the Notch pathway are involved in each signalling process. Using clonal analysis we show that a ligand of Notch, Delta, is required along with Notch for both proneural enhancement and lateral specification, while the downstream components Suppressor-of-Hairless and Enhancer-of-Split are involved only in lateral specification. Our data point to a distinct signal transduction pathway during proneural enhancement by Notch. Using misexpression experiments we also show that particular Enhancer-of-split bHLH genes can differ greatly in their contribution to lateral specification.