Impact of combined resistance and aerobic exercise training on branched-chain amino acid turnover, glycine metabolism and insulin sensitivity in overweight humans.
Impact of combined resistance and aerobic exercise training on branched-chain amino acid turnover, glycine metabolism and insulin sensitivity in overweight humans.
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DOI：
10.1007/s00125-015-3705-6
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发表时间：
2015-10
期刊：
影响因子：
8.2
通讯作者：
Kraus WE
中科院分区：
文献类型：
--
作者：
Glynn EL;Piner LW;Huffman KM;Slentz CA;Elliot-Penry L;AbouAssi H;White PJ;Bain JR;Muehlbauer MJ;Ilkayeva OR;Stevens RD;Porter Starr KN;Bales CW;Volpi E;Brosnan MJ;Trimmer JK;Rolph TP;Newgard CB;Kraus WE
肥胖与胰岛素敏感性（IS）降低和血浆支链氨基酸（BCAA）升高有关。本研究旨在探讨运动干预过程中超重者支链氨基酸代谢与胰岛素抵抗的关系。在10名健康对照（对照）和9名OW、未经训练的胰岛素抵抗个体（OW-未经训练）中测量全身亮氨酸周转、高胰岛素-正常血钳夹IS、循环和骨骼肌氨基酸、支链α-酮酸和酰基肉毒碱。OW-未经训练，然后进行6个月的有氧和阻力运动计划和重复测试（OW-训练）。对照组的IS高于OW-未经训练组，并且在运动后显著增加。相对于体重，OW训练组的IS低于对照组，但当标准化为无脂肪质量（FFM）时，与对照组无差异。血浆BCAA和亮氨酸周转（相对于FFM）在OW-未经训练的与对照组相比更高，但平均运动没有变化。尽管如此，在个体中，循环中支链氨基酸摩尔总和的减少是IS改善的最佳代谢预测因子。对照组和OW-训练组的循环甘氨酸水平高于OW-未训练组，尿代谢分析表明，运动通过形成尿甘氨酸加合物诱导更有效地消除BCAA和芳香族氨基酸（AA）代谢产生的过量酰基。一种机制，涉及更有效地消除多余的酰基来自支链氨基酸和芳香族AA代谢通过甘氨酸共轭在肝脏中，而不是增加支链氨基酸处置通过氧化和营业额，可能介导运动，支链氨基酸代谢和IS之间的相互作用。Clinicaltrials.gov www.example.com
Obesity is associated with decreased insulin sensitivity (IS) and elevated plasma branched-chain amino acids (BCAAs). The purpose of this study was to investigate the relationship between BCAA metabolism and IS in overweight (OW) individuals during exercise intervention. Whole-body leucine turnover, IS by hyperinsulinaemic–euglycaemic clamp, and circulating and skeletal muscle amino acids, branched-chain α-keto acids and acylcarnitines were measured in ten healthy controls (Control) and nine OW, untrained, insulin-resistant individuals (OW-Untrained). OW-Untrained then underwent a 6 month aerobic and resistance exercise programme and repeated testing (OW-Trained). IS was higher in Control vs OW-Untrained and increased significantly following exercise. IS was lower in OW-Trained vs Control expressed relative to body mass, but was not different from Control when normalised to fat-free mass (FFM). Plasma BCAAs and leucine turnover (relative to FFM) were higher in OW-Untrained vs Control, but did not change on average with exercise. Despite this, within individuals, the decrease in molar sum of circulating BCAAs was the best metabolic predictor of improvement in IS. Circulating glycine levels were higher in Control and OW-Trained vs OW-Untrained, and urinary metabolic profiling suggests that exercise induces more efficient elimination of excess acyl groups derived from BCAA and aromatic amino acid (AA) metabolism via formation of urinary glycine adducts. A mechanism involving more efficient elimination of excess acyl groups derived from BCAA and aromatic AA metabolism via glycine conjugation in the liver, rather than increased BCAA disposal through oxidation and turnover, may mediate interactions between exercise, BCAA metabolism and IS. Clinicaltrials.gov NCT01786941