Differential development of TRPV1-expressing sensory nerves in peripheral organs
Differential development of TRPV1-expressing sensory nerves in peripheral organs
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DOI：
10.1007/s00441-005-0013-3
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发表时间：
2006-01-01
影响因子：
3.6
通讯作者：
Yazama, F
中科院分区：
文献类型：
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作者：
Funakoshi, K;Nakano, M;Yazama, F
在小鼠个体发育中，在胚胎第 13 天 (E13) 时，主要在背根神经节 (DRG) 中观察到对瞬时受体电位香草酸受体 1 (TRPV1) 具有免疫反应的神经元。胚胎期TRPV1(+)神经元数量减少，但出生后逐渐增加。一些TRPV1(+)神经元对降钙素基因相关肽(CGRP)也有免疫反应。出生后第7天（P7），L4 DRG中66％的CGRP（+）神经元为TRPV1（+），55％的TRPV1（+）神经元也是CGRP（+）。在外周器官中，E14时在皮肤中短暂观察到TRPV1免疫反应性神经纤维。在 E14 的泌尿道和 E15 的直肠中也观察到了它们。这些器官中的许多TRPV1(+)神经纤维也是CGRP(+)。 P1时，在呼吸器官中观察到TRPV1(+)神经纤维，在胃、结肠、皮肤和骨骼肌中观察到较小程度的TRPV1(+)神经纤维。出生后各器官上TRPV1(+)神经纤维的数量逐渐增加。 P7时，在小肠和肾脏中也观察到TRPV1(+)神经纤维。在大多数器官中，P7 时与 CGRP 共定位的总 TRPV1(+) 神经纤维的百分比高于 P1 时的百分比。目前的结果表明，TRPV1 在外周过程中的表达在器官之间存在差异。 TRPV1在细胞体中表达的差异时间过程可能与其投射到的器官有关。 TRPV1 与 CGRP 在神经纤维上的共定位在器官之间也存在差异。这表明在某些病理生理条件下发生的 TRPV1 介导的神经肽释放在器官之间也存在差异。
In mouse ontogeny, neurons immunoreactive for transient receptor potential vanilloid receptor 1 (TRPV1) were observed primarily in the dorsal root ganglia (DRG) at embryonic day 13 (E13). In the embryonic period, the number of TRPV1(+) neurons decreased, but then gradually increased postnatally. Some of TRPV1(+) neurons were also immunoreactive for calcitonin gene-related peptide (CGRP). At postnatal day 7 (P7), 66% of CGRP(+) neurons were TRPV1(+), and 55% of TRPV1(+) neurons were also CGRP(+) in the L4 DRG. In the peripheral organs, TRPV1-immunoreactive nerve fibers were transiently observed in the skin at E14. They were also observed in the urinary tract at E14, and in the rectum at E15. Many TRPV1(+) nerve fibers in these organs were also CGRP(+). At P1, TRPV1(+) nerve fibers were observed in the respiratory organs, and to a lesser extent in the stomach, colon, skin, and skeletal muscles. The number of TRPV1(+) nerve fibers on each organ gradually increased postnatally. At P7, TRPV1(+) nerve fibers were also observed in the small intestine and kidneys. The percentage of total TRPV1(+) nerve fibers that co-localized with CGRP was greater in most organs at P7 than at P1. The present results indicate that TRPV1 expression on peripheral processes differs among organs. The differential time course of TRPV1 expression in the cell bodies might be related to the organs to which they project. Co-localization of TRPV1 with CGRP on nerve fibers also varies among organs. This suggests that the TRPV1-mediated neuropeptide release that occurs in certain pathophysiologic conditions also varies among organs.