Molecular Characterization of Begomoviruses Associated with Yellow Leaf Curl Disease in Solanaceae and Cucurbitaceae Crops from Northern Sumatra, Indonesia
Molecular Characterization of Begomoviruses Associated with Yellow Leaf Curl Disease in Solanaceae and Cucurbitaceae Crops from Northern Sumatra, Indonesia
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DOI：
10.2503/hortj.utd-175
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Koeda, Sota
中科院分区：
文献类型：
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作者：
Kesumawati, Elly;Okabe, Shoko;Koeda, Sota
由烟粉虱（Bemisia tabaci）传播的贝戈莫病毒已成为全世界多种蔬菜作物种植的严重限制因素。在印度尼西亚亚齐省北苏门答腊岛采集了表现出典型贝戈莫病毒黄化和/或卷曲症状的茄科（番茄、烟草和茄子）和葫芦科（黄瓜和南瓜）植物的叶子样本。使用从黄瓜、南瓜、茄子和烟草中分离的 DNA 进行滚环扩增，并对 Begomovirus 的全长序列进行评估。分离出以下病毒：二分叶戈莫病毒新德里番茄卷叶病毒（ToLCNDV）、南瓜卷叶中国病毒（SLCCNV）、北碧府番茄黄叶曲叶病毒（TYLCKaV）和单叶叶戈莫病毒Ageratum黄脉病毒（AYVV）。 Begomovirus 诊断是通过 PCR 使用 Begomovirus 物种特异性引物进行的，该引物针对印度尼西亚胡椒黄曲叶病毒 (PepYLCIV)、亚齐胡椒黄曲叶病毒 (PepYLCAV)、ToLCNDV、SLCCNV、TYLCKaV 和 AYVV（这些主要的 Begomo 病毒）。每种植物的主要 Begomovirus 种类如下：番茄 PepYLCAV、烟草 AYVV、茄子 TYLCKaV、黄瓜 ToLCNDV 和南瓜 SLCCNV。这项研究为园艺作物育种抗 Begomovirus 品种提供了有价值的信息。
Begomoviruses, transmitted by whiteflies (Bemisia tabaci), have emerged as serious constraints to the cultivation of a wide variety of vegetable crops worldwide. Leaf samples from Solanaceae (tomato, tobacco, and eggplant) and Cucurbitaceae (cucumber and squash) plants exhibiting typical begomoviral yellowing and/or curling symptoms were collected in Northern Sumatra, Aceh province, Indonesia. Rolling circle amplification was conducted using DNA isolated from cucumber, squash, eggplant, and tobacco, and the full-length sequences of the begomoviruses were evaluated. The following viruses were isolated: bipartite begomoviruses Tomato leaf curl New Delhi virus (ToLCNDV), Squash leaf curl China virus (SLCCNV), Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), and a monopartite begomovirus Ageratum yellow vein virus (AYVV). Begomovirus diagnosis was conducted by PCR using begomovirus species-specific primers for Pepper yellow leaf curl Indonesia virus (PepYLCIV), Pepper yellow leaf curl Aceh virus (PepYLCAV), ToLCNDV, SLCCNV, TYLCKaV, and AYVV, which are the predominant begomoviruses. The primary begomovirus species for each plant were as follows: PepYLCAV for tomato, AYVV for tobacco, TYLCKaV for eggplant, ToLCNDV for cucumber, and SLCCNV for squash. This study provides valuable information for breeding begomovirus-resistant cultivars as horticultural crops.