Antagonistic gene transcripts regulate adaptation to new growth environments
Antagonistic gene transcripts regulate adaptation to new growth environments
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DOI：
10.1073/pnas.1111408109
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发表时间：
2011-12-27
影响因子：
11.1
通讯作者：
Hasty, Jeff
中科院分区：
文献类型：
--
作者：
Baumgartner, Bridget L.;Bennett, Matthew R.;Hasty, Jeff
细胞已经进化出复杂的调节网络，重组基因表达模式以响应不断变化的环境条件。这些变化通常涉及影响不同水平基因表达的冗余机制。在这里，我们研究了酿酒酵母半乳糖利用网络中mRNA降解增强的后果。我们观察到葡萄糖诱导的GAL1转录物降解在添加葡萄糖后为细胞提供了短暂的生长优势。我们发现这种优势来自于GAL1转录本和细胞周期蛋白CLN3转录本之间的翻译竞争，CLN3是一种翻译调节的细胞分裂启动物。这种竞争造成了翻译瓶颈，平衡了Gal1p和Cln3p的产生，并代表了一种增强细胞适应碳源变化能力的转录后控制机制。我们提供的证据表明，GAL1和CLN3转录本的空间调节是在葡萄糖可用性波动期间维持生长的原因。我们的结果为细胞在动态环境中生长过程中如何优化能量使用提供了独特的见解。
Cells have evolved complex regulatory networks that reorganize gene expression patterns in response to changing environmental conditions. These changes often involve redundant mechanisms that affect various levels of gene expression. Here, we examine the consequences of enhanced mRNA degradation in the galactose utilization network of Saccharomyces cerevisiae. We observe that glucose-induced degradation of GAL1 transcripts provides a transient growth advantage to cells upon addition of glucose. We show that the advantage arises from relief of translational competition between GAL1 transcripts and those of cyclin CLN3, a translationally regulated initiator of cell division. This competition creates a translational bottleneck that balances the production of Gal1p and Cln3p and represents a posttranscriptional control mechanism that enhances the cell's ability to adapt to changes in carbon source. We present evidence that the spatial regulation of GAL1 and CLN3 transcripts is what allows growth to be maintained during fluctuations of glucose availability. Our results provide unique insights into how cells optimize energy use during growth in a dynamic environment.