INVITRO BIOSYNTHESIS OF BETA-ENDORPHIN IN PITUITARY-GLANDS
INVITRO BIOSYNTHESIS OF BETA-ENDORPHIN IN PITUITARY-GLANDS
复制标题
DOI：
10.1073/pnas.74.4.1403
复制
发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
CHRETIEN, M
中科院分区：
文献类型：
--
作者：
CRINE, P;BENJANNET, S;CHRETIEN, M
度量。内啡肽是一种由31个氨基酸组成的多肽，从不同种类动物的脑垂体中分离出来。它有很强的吗啡样活性。它是由- β -促脂蛋白的61-91氨基酸残基组成的。人们猜测它是原位生物合成的还是β -促脂蛋白分泌后转化的。目前的体外研究表明，它被发现为。β。与放射性氨基酸前体[35S]蛋氨酸孵育的牛垂体切片中的-内啡肽。化学表征和微测序的新生物合成材料证明了其与分离的未标记β的身份。-内啡肽，并表明它在第5位有蛋氨酸残基，正如预期的那样。
.beta.-Endorphin is a 31 amino acid polypeptide isolated from the pituitary gland of different species of animals. It has strong morphine-like activity. It is formed of amino acid residues 61-91 of .beta.-lipotropin. Speculation has arisen whether it is biosynthesized in situ or transformed after secretion of .beta.-lipotropin. The present in vitro studies show that it is found as .beta.-endorphin in bovine pituitary slices incubated with radioactive amino acid precursor [35S]methionine. Chemical characterization and microsequencing of a newly biosynthesized material proves its identity with isolated unlabeled .beta.-endorphin and shows that it has a methionine residue at its 5th position, as expected.