Optogenetics enables real-time spatiotemporal control over spiral wave dynamics in an excitable cardiac system
Optogenetics enables real-time spatiotemporal control over spiral wave dynamics in an excitable cardiac system
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DOI：
10.1101/413658
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发表时间：
2018-09
期刊：
影响因子：
7.7
通讯作者：
R. Majumder;I. Feola;A. Teplenin;A. D. de Vries;A. Panfilov;D. Pijnappels
中科院分区：
文献类型：
--
作者：
R. Majumder;I. Feola;A. Teplenin;A. D. de Vries;A. Panfilov;D. Pijnappels
非线性波的传播是不同生物系统功能的关键。这样的波浪可以组织成螺旋形，围绕核心旋转，其属性决定了整体的波浪动力学。从理论上讲，操纵螺旋波核心应该导致对其动力学的完全时空控制。然而，这一理论缺乏支持性证据（即使在概念层面），因此它是一个长期存在的假设。在这里，我们提出了一个新的现象学概念，涉及人工拖动螺旋波的核心，以证明上述假设在硅片，随后在体外验证在大鼠心房心肌细胞的光遗传学修饰的单层。因此，我们连接以前建立的，但不相关的概念螺旋波的吸引力，锚定和unpinning证明，核心操纵，通过控制位移的不均匀性在可激发介质中，允许螺旋波沿着预定义的轨迹强制运动。因此，我们施加实时时空控制在生物系统中的螺旋波动力学。
Propagation of non-linear waves is key to the functioning of diverse biological systems. Such waves can organize into spirals, rotating around a core, whose properties determine the overall wave dynamics. Theoretically, manipulation of a spiral wave core should lead to full spatiotemporal control over its dynamics. However, this theory lacks supportive evidence (even at a conceptual level), making it thus a long-standing hypothesis. Here, we propose a new phenomenological concept that involves artificially dragging spiral waves by their cores, to prove the afore-mentioned hypothesis in silico, with subsequent in vitro validation in optogenetically-modified monolayers of rat atrial cardiomyocytes. We thereby connect previously established, but unrelated concepts of spiral wave attraction, anchoring and unpinning to demonstrate that core manipulation, through controlled displacement of heterogeneities in excitable media, allows forced movement of spiral waves along pre-defined trajectories. Consequently, we impose real-time spatiotemporal control over spiral wave dynamics in a biological system.