DNA Content Differences Between Male and Female Chicken (Gallus gallus domesticus) Nuclei and Z and W Chromosomes Resolved by Image Cytometry
DNA Content Differences Between Male and Female Chicken (Gallus gallus domesticus) Nuclei and Z and W Chromosomes Resolved by Image Cytometry
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DOI：
10.1369/jhc.2009.954727
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发表时间：
2010-03-01
影响因子：
3.2
通讯作者：
Clarindo, Wellington Ronildo
中科院分区：
文献类型：
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作者：
Correa Mendonca, Maria Andreia;Carvalho, Carlos Roberto;Clarindo, Wellington Ronildo
鸡红细胞（CRBC）被广泛用作DNA含量测定的标准品。细胞遗传学数据表明，Z性染色体大约是W性染色体的两倍大，因此雄性（ZZ）和雌性（ZW）鸡之间的DNA含量存在一定程度的差异。尽管如此，男性和女性CRBC已不加区别地用于绝对基因组大小测定。我们的工作是为了验证是否可以通过图像细胞术（ICM）来分辨雄性和雌性原鸡“来航”细胞核和ZZ/ZW染色体之间的DNA含量差异。使用通过Feulgen反应染色的风干涂片进行细胞核分析。鸡中期铺展后Feulgen染色进行了分析，以获得Z和W染色体的定量信息。在每次捕获会话之前，我们对ICM仪器进行质量控制。我们的结果从核测量表明，2C值是0.09 pg的男性比女性高。在染色体中，我们发现Z染色体的DNA含量比W染色体多200%。ICM表现出区分基因组中低DNA含量差异的分辨率。我们建议在比较性细胞分析中一般使用CRBC 2C值作为标准时应谨慎。（J Histochem Cytochem 58：229-235，2010）
Chicken red blood cells (CRBCs) are widely used as standards for DNA content determination. Cytogenetic data have shown that the Z sex chromosome is approximately twice as large as the W, so that the DNA content differs to some extent between male (ZZ) and female (ZW) chickens. Despite this fact, male and female CRBCs have been indiscriminately used in absolute genome size determination. our work was conducted to verify whether the DNA content differences between male and female Gallus gallus domesticus "Leghorn" nuclei and ZZ/ZW chromosomes can be resolved by image cytometry (ICM). Air-dried smears stained by Feulgen reaction were used for nuclei analysis. Chicken metaphase spreads upon Feulgen staining were analyzed for obtaining quantitative information on the Z and W chromosomes. Before each capture session, we conducted quality control of the ICM instrumentation. Our results from nuclear measurements showed that the 2C value is 0.09 pg higher in males than in females. In chromosomes, we found that the Z chromosome shows 200% more DNA content than does the W chromosome. ICM demonstrated resolution power to discriminate low DNA content differences in genomes. We suggest prudence in the general use of CRBC 2C values as standards in comparative cytometric analysis. (J Histochem Cytochem 58:229-235, 2010)