Synthesis and Characterization of Au102(p-MBA)44 Nanoparticles
Synthesis and Characterization of Au102(p-MBA)44 Nanoparticles
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DOI：
10.1021/ja109131w
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发表时间：
2011-03-09
影响因子：
15
通讯作者：
Kornberg, Roger D.
中科院分区：
文献类型：
--
作者：
Levi-Kalisman, Yael;Jadzinsky, Pablo D.;Kornberg, Roger D.
描述了制备规模的高收率的Au-102（p-MBA）（44）纳米颗粒的合成。各种分析方法显示出与颗粒的组成和已知结构一致的结果，表明制备基本上是均匀的，并且也证明了方法的有效性。证明了蛋白质和DNA的颗粒的衍生化，并描述了通过低温EM在低电子剂量下成像单个颗粒的条件，接近焦点，记录高分辨率细节的最佳条件。
The synthesis of Au-102(p-MBA)(44) nanoparticles on a preparative scale in high yield is described. Various analytical methods are shown to give results consistent with the composition and known structure of the particles, showing the preparation is essentially homogeneous, and attesting to the validity of the methods as well. Derivatization of the particles with proteins and DNA is demonstrated, and conditions are described for imaging individual particles by cryo-EM at low electron dose, close to focus, conditions optimal for recording high-resolution details.