Transposon Tn5 mutagenesis of Brucella abortus.
Transposon Tn5 mutagenesis of Brucella abortus.
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流产布鲁氏菌的转座子 Tn5 诱变。
DOI：
10.1128/iai.55.11.2774-2776.1987
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发表时间：
1987
影响因子：
3.1
通讯作者：
Heffron,F
中科院分区：
文献类型：
--
作者：
Smith,LD;Heffron,F
我们证明转座子介导的诱变可以用于构建流产布鲁氏菌的突变。我们使用质粒和噬菌体将Tn5或Tn5 lac引入布鲁氏菌染色体。B. abortus天生对卡那霉素敏感。我们在菌株US-19和菌株2308中分别选择了22个独立的卡那霉素耐药菌落。我们已经通过Southern杂交证明Tn5被插入到染色体中。
We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 22 independent kanamycin-resistant colonies in strain US-19 and 19 colonies in strain 2308. We have demonstrated by Southern hybridization that Tn5 was inserted into the chromosome.