In vitro effects of cadmium chloride on preimplantation rat embryos.
In vitro effects of cadmium chloride on preimplantation rat embryos.
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氯化镉对植入前大鼠胚胎的体外影响。
DOI：
10.1016/0147-6513(86)90013-8
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发表时间：
1986
影响因子：
6.8
通讯作者：
Ashok,L
中科院分区：
文献类型：
--
作者：
Abraham,R;Charles,AK;Mankes,R;LeFevre,R;Renak,V;Ashok,L
将植入前的大鼠胚胎(8-细胞、桑椹胚、囊胚期)在含或不含氯化镉(1μg/ml)的Brinster培养液中培养24 h。氯化镉抑制了8-细胞和桑椹胚囊胚的发育。形态观察显示，8-细胞胚胎细胞缩小，核固缩。与可比对照相比，桑叶的体积更小，细胞更少。氯化镉对早期囊胚有明显的细胞毒性，内团细胞损伤最严重，细胞死亡、固缩、碎屑堆积。
Preimplantation rat embryos (8-cell, morulae, blastocysts) were incubated for 24 hrin vitroin a Brinster medium with or without cadmium chloride (1 μg/ml). CdCl2arrested the development of both the 8-cell and morulae into blastocysts. Morphologic observations revealed the 8-cell embryo to have shrunken cells and pyknotic nuclei. Morulae appeared smaller in size with fewer cells than comparable controls. CdCl2was clearly cytotoxic to the early blastocysts, inner mass cells were most damaged with cell death, pyknosis, and accumulation of debris.