nrdD and nrdG genes are essential for strict anaerobic growth of Escherichia coli
nrdD and nrdG genes are essential for strict anaerobic growth of Escherichia coli
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DOI：
10.1006/bbrc.1996.1778
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发表时间：
1996-12-04
影响因子：
3.1
通讯作者：
Reichard, P
中科院分区：
文献类型：
--
作者：
Garriga, X;Eliasson, R;Reichard, P
在大肠杆菌中，在好氧和厌氧生长过程中，核苷酸还原是由两种不同的酶催化的。好氧酶由nrdAB基因编码，厌氧酶由nrdDG编码。我们现在证明，nrdD或nrdG的敲除突变株在严格的厌氧条件下不能生长，这是通过在培养基中加入硫化钠实现的。有趣的是，这些突变株在微需氧条件下通过过量生产需氧酶而生长良好。在这种情况下，野生型细菌关闭nrdAB，打开nrdDG。(C)1996年学术出版社。
In Escherichia coli ribonucleotide reduction is catalyzed by two separate enzymes during aerobic and anaerobic growth. The aerobic enzyme is coded by the nrdAB genes, the anaerobic enzyme by nrdDG. We now show that knock-out mutants of either nrdD or nrdG cannot grow during strict anaerobiosis, achieved by inclusion of sodium sulfide in the medium. Interestingly, these mutants grow well under microaerophilic conditions by overproducing the aerobic enzyme. Under such conditions wild-type bacteria turn off nrdAB and switch on nrdDG. (C) 1996 Academic Press, Inc.