Vδ1 T lymphocytes producing IFN-γ and IL-17 are expanded in HIV-1-infected patients and respond to Candida albicans
Vδ1 T lymphocytes producing IFN-γ and IL-17 are expanded in HIV-1-infected patients and respond to Candida albicans
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DOI：
10.1182/blood-2009-01-198028
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发表时间：
2009-06-25
期刊：
影响因子：
20.3
通讯作者：
Zocchi, Maria Raffaella
中科院分区：
文献类型：
--
作者：
Fenoglio, Daniela;Poggi, Alessandro;Zocchi, Maria Raffaella
在早期HIV-1感染中，外周血中Vδ1T淋巴细胞增加，这与趋化因子受体表达、趋化因子反应和再循环有关。在此，我们表明，与健康供体不同，在 HIV-1 感染患者中，离体分离的 V delta 1 T 细胞显示细胞质干扰素-γ (IFN-γ)。有趣的是，这些细胞共表达细胞质白细胞介素 17 (IL-17)，并带有记忆 T 细胞亚群的 CD27 表面标记。从患者或健康供体中分离出来的 V δ 1 T 细胞可以在体外对白色念珠菌作出反应，增殖并产生 IFN-γ 和 IL-17，而 V δ 2 T 细胞则对分枝杆菌或磷酸盐抗原进行增殖和产生 IFN-γ/IL-17 反应。这些 IFN-gamma/IL-17 双生产者 γ δ T 细胞表达 Th17 RORC 和 Th1 TXB21 转录因子，并具有参与 γ δ T 细胞跨内皮迁移的 CCR7 归巢受体和 CD161 分子。此外，响应白色念珠菌的 V delta 1 T 细胞表达趋化因子受体 CCR4 和 CCR6。这种专门配备的循环记忆γδT细胞群可能在控制HIV-1传播和防御机会性感染方面发挥重要作用，可能有助于补偿CD4(+) T细胞的损伤。 （血。2009；113：6611-6618）
In early HIV-1 infection, V delta 1 T lymphocytes are increased in peripheral blood and this is related to chemokine receptor expression, chemokine response, and recirculation. Herein we show that, at variance with healthy donors, in HIV-1-infected patients ex vivo-isolated V delta 1 T cells display cytoplasmic interferon-gamma (IFN-gamma). Interestingly, these cells coexpress cytoplasmic interleukin-17 (IL-17), and bear the CD27 surface marker of the memory T-cell subset. V delta 1 T cells, isolated from either patients or healthy donors, can proliferate and produce IFN-gamma and IL-17 in response to Candida albicans in vitro, whereas V delta 2 T cells respond with proliferation and IFN-gamma/IL-17 production to mycobacterial or phosphate antigens. These IFN-gamma/IL-17 double-producer gamma delta T cells express the Th17 RORC and the Th1 TXB21 transcription factors and bear the CCR7 homing receptor and the CD161 molecule that are involved in gamma delta T-cell transendothelial migration. Moreover, V delta 1 T cells responding to C albicans express the chemokine receptors CCR4 and CCR6. This specifically equipped circulating memory gamma delta T-cell population might play an important role in the control of HIV-1 spreading and in the defense against opportunistic infections, possibly contributing to compensate for the impairment of CD4(+) T cells. (Blood. 2009; 113: 6611-6618)