Comparative structural modeling and inference of conserved protein classes in Drosophila seminal fluid.
Comparative structural modeling and inference of conserved protein classes in Drosophila seminal fluid.
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DOI：
10.1073/pnas.0405579101
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发表时间：
2004-09
影响因子：
11.1
通讯作者：
Jacob L. Mueller;D. Ripoll;C. Aquadro;M. Wolfner
中科院分区：
文献类型：
--
作者：
Jacob L. Mueller;D. Ripoll;C. Aquadro;M. Wolfner
精液的成分是蛋白质和其他分子的复杂混合物，其大部分功能尚未确定，其中许多正在迅速进化。为了阐明这些蛋白质的作用并揭示它们快速进化所隐藏的潜在功能相似性，我们对果蝇雄性附腺产生的52个预测精液蛋白质中的28个进行了比较结构建模。每个模型的特点是定义了可能对结构和功能重要的残基。将已知的蛋白质结构与预测的副腺蛋白(ACP)进行比较，发现了初级序列比对无法检测到的相似之处。这些结构预测ACPs可分为几类：蛋白分解调节因子、脂质调节剂、免疫/保护因子、精子结合蛋白和多肽激素。比较结构建模方法表明，哺乳动物和果蝇精液蛋白的主要功能类别是保守的，尽管生殖策略不同。面对包括果蝇和哺乳动物精液蛋白在内的许多生殖蛋白的快速蛋白质序列进化，这一点尤其令人震惊。
The constituents of seminal fluid are a complex mixture of proteins and other molecules, most of whose functions have yet to be determined and many of which are rapidly evolving. As a step in elucidating the roles of these proteins and exposing potential functional similarities hidden by their rapid evolution, we performed comparative structural modeling on 28 of 52 predicted seminal proteins produced in the Drosophila melanogaster male accessory gland. Each model was characterized by defining residues likely to be important for structure and function. Comparisons of known protein structures with predicted accessory gland proteins (Acps) revealed similarities undetectable by primary sequence alignments. The structures predict that Acps fall into several categories: regulators of proteolysis, lipid modifiers, immunity/protection, sperm-binding proteins, and peptide hormones. The comparative structural modeling approach indicates that major functional classes of mammalian and Drosophila seminal fluid proteins are conserved, despite differences in reproductive strategies. This is particularly striking in the face of the rapid protein sequence evolution that characterizes many reproductive proteins, including Drosophila and mammalian seminal proteins.