A novel homeobox cluster expressed in repeated structures of the midgut.
A novel homeobox cluster expressed in repeated structures of the midgut.
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DOI：
10.1006/dbio.1995.1294
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发表时间：
1995-10
影响因子：
2.7
通讯作者：
J. Wysocka-Diller;G. Aisemberg;E. Macagno
中科院分区：
文献类型：
--
作者：
J. Wysocka-Diller;G. Aisemberg;E. Macagno
水蛭中含有三个重复同源盒序列（Lox3A、Lox3B 和 Lox3C）的基因簇（LOX3-C）已被鉴定。水蛭同源盒与触角足类基因具有有限的同源性，但在目前表征的昆虫基因中不具有同源性。 Lox3基因属于一个名为Xlox的新家族，该家族还包括来自小鼠、大鼠、青蛙和远亲水蛭Helobdella triserialis的基因。 Xlox 家族的所有成员都在胚胎肠道的特定区域表达，它们似乎影响形态发生和细胞分化。这里描述的 LOX3-C 的三个同源框包含在几乎相同的直接串联重复序列中。 LOX3-C 区域至少产生两种转录本，一种存在于胚胎和成体中，另一种仅存在于早期胚胎中。早期Lox3表达仅限于中肠原基的特定区域，在中肠将收缩形成嗉囊的11个憩室的位置发现的12个分段重复的梭状细胞横向条纹中。提出了 LOX3-C 在最初均质中肠中分段迭代结构发展中的作用。
A gene cluster (LOX3-C) containing three duplicated homeobox sequences (Lox3A, Lox3B, and Lox3C) was characterized in the leech Hirudo medicinalis. The leech homeoboxes have a limited homology to those of Antennapedia-class genes, but do not have homologs among currently characterized insect genes. The Lox3 genes belong to a new family, named Xlox, that also includes genes from mouse, rat, frog, and a distantly related leech, Helobdella triserialis. All members of the Xlox family are expressed in specific regions of the embryonic gut, where they seem to affect morphogenesis and cell differentiation. The three homeoboxes of LOX3-C described here are contained within nearly identical direct tandem repeats. The LOX3-C region produces at least two transcripts, one present in both embryos and adults and the other only in early embryos. Early Lox3 expression is restricted to specific regions of the midgut primordium, in 12 segmentally repeated, transverse stripes of fusiform cells found at the positions where the midgut will constrict to form the 11 diverticula of the crop. A role in the development of segmentally iterated structures in an initially homogeneous midgut is proposed for LOX3-C.