Multiplex PCR for 18 X-chromosomal STRs in Japanese population
Multiplex PCR for 18 X-chromosomal STRs in Japanese population
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DOI：
10.1016/j.legalmed.2012.10.006
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发表时间：
2013-05-01
期刊：
影响因子：
1.5
通讯作者：
Minaguchi, Kiyoshi
中科院分区：
文献类型：
--
作者：
Nakamura, Yasutaka;Samejima, Michinaga;Minaguchi, Kiyoshi
x染色体STR （X-STR）多态性在涉及通过女性受试者遗传的亲属关系检测的复杂情况下特别有用。本研究建立并优化了单次PCR扩增18个STRs的x染色体多重链。多路系统包括DXS7424、GATA172D05、HPRTB、DXS8377、GATA31E08、DXS6810、DXS7423、DXS981、DXS6795、DXS6803、DXS6789、DXS6800、DXS6809、DXS7133、DXS7132、DXS9902、DXS101和DXS6807位点，分布在整个x染色体上。它被设计为确定亲属关系检测中整个X染色体重组的潜在第一选择。等位基因频率来自378名日本男性和175名日本女性，所有人都不相关。扩增产物大小在82 ~ 297 bp之间。18个位点的女性和男性的总分辨力分别为0.99999999999999997和0.9999999992。提出了一个案例，在这个案例中，该系统在达成解决方案方面具有相当大的效力。在测试的X-STR多路系统中，目前的多路系统扩增的基因座数量最多，表明其在个人识别和确定亲属关系方面的潜力。2012爱思唯尔爱尔兰有限公司版权所有。
X-chromosomal STR (X-STR) polymorphisms are particularly useful in complex cases of kinship testing involving inheritance through female subjects. An X-chromosomal multiplex amplifying 18 STRs in one single PCR reaction was developed and optimized in this study. The multiplex system included the DXS7424, GATA172D05, HPRTB, DXS8377, GATA31E08, DXS6810, DXS7423, DXS981, DXS6795, DXS6803, DXS6789, DXS6800, DXS6809, DXS7133, DXS7132, DXS9902, DXS101 and DXS6807 loci, which are distributed over the whole X-chromosome. It was designed as a potential first option in determining recombination within the whole X chromosome in kinship testing. Allele frequencies were obtained from samples from 378 male and 175 female Japanese individuals, all unrelated. The sizes of the amplified products ranged from 82 to 297 bp. The combined power of discrimination of the 18 loci was 0.999999999999997 in females and 0.9999999992 in males. A case is presented in which this system allowed considerable efficacy in reaching a solution. The present multiplex system amplified the largest number of loci among the X-STR multiplex systems tested, indicating its potential in personal identification and determining kinship. (C) 2012 Elsevier Ireland Ltd. All rights reserved.