Quantitative trait locus analysis for pod- and kernel-related traits in the cultivated peanut (Arachis hypogaea L.).
Quantitative trait locus analysis for pod- and kernel-related traits in the cultivated peanut (Arachis hypogaea L.).
复制标题
栽培花生 (Arachishypogaea L.) 荚果和仁相关性状的数量性状位点分析
DOI：
10.1186/s12863-016-0337-x
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发表时间：
2016-01-25
期刊：
影响因子：
2.9
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Chen W;Jiao Y;Cheng L;Huang L;Liao B;Tang M;Ren X;Zhou X;Chen Y;Jiang H
背景：花生（Arachis hypogaea L.）是世界上重要的油料和粮食作物。与荚果和籽粒相关的性状是决定花生产量的直接因素。然而，花生荚果和籽粒相关性状的遗传基础在很大程度上仍不清楚，这阻碍了通过分子标记辅助选择来改良花生。为了解花生荚果和籽粒相关性状的遗传基础，为花生分子标记辅助育种提供更多有用信息，本研究以富川大花生× ICG 6375的两个F2：3群体为材料，对荚果长、宽和籽粒长、宽进行了QTL分析（FI群体）和品种徐花13 ×品种中花6（XZ人群）。结果：构建了FI和XZ群体的遗传图谱，分别包含347和228个多态性标记。共检测到39个QTL，可解释1.25-26.11%的表型变异。在FI群体中，共检测到26个QTL，其中12个未定位。在XZ群体中共检测到13个QTL，其中8个QTL未见报道。结论：本研究利用两个作图群体对荚长、荚宽和种子长、荚宽进行了QTL分析。在F1群体中检测到2个影响角果长、4个影响角果宽、5个影响粒长和1个影响粒宽的QTL，在XZ群体中检测到3个影响角果长、3个影响角果宽和2个影响粒宽的QTL。本研究结果将有助于花生荚果和种子相关性状的分子标记辅助选择。
Background:The cultivated peanut (Arachis hypogaea L.) is an important oil and food crop in the world. Pod- and kernel-related traits are direct factors involved in determining the yield of the peanut. However, the genetic basis underlying pod- and kernel-related traits in the peanut remained largely unknown, which hampered the improvement of peanut through marker-assisted selection. To understand the genetic basis underlying pod- and kernel-related traits in the peanut and provide more useful information for marker-assisted breeding, we conducted quantitative trait locus (QTL) analysis for pod length and width and seed length and width by use of two F2:3 populations derived from cultivar Fuchuan Dahuasheng × ICG 6375 (FI population) and cultivar Xuhua 13 × cultivar Zhonghua 6 (XZ population) in this study.Results:Two genetic maps containing 347 and 228 polymorphic markers were constructed for FI and XZ populations respectively. In total, 39 QTLs explaining 1.25-26.11% of the phenotypic variations were detected in two populations. For the FI population, 26 QTLs were detected between the two environments, among which twelve were not mapped before. For the XZ population, thirteen QTLs were detected, among which eight were not reported before. One QTL for pod width was repeatedly mapped between the two populations.Conclusion:The QTL analyses for pod length and width and seed length and width were conducted in this study using two mapping populations. Novel QTLs were identified, which included two for pod length, four for pod width, five for seed length and one for seed width in the FI population, and three for pod length, three for pod width and two for seed width in the XZ population. Our results will be helpful for improving pod- and seed-related traits in peanuts through marker-assisted selection.