Temperature-sensitive and circadian oscillators of Neurospora crassa share components.
Temperature-sensitive and circadian oscillators of Neurospora crassa share components.
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DOI：
10.1534/genetics.111.137976
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发表时间：
2012-05
期刊：
影响因子：
3.3
通讯作者：
Heintzen C
中科院分区：
文献类型：
--
作者：
Hunt S;Elvin M;Heintzen C
在粗糙脉孢菌中，频率（frq）、频率相互作用RNA解旋酶（frh）、白色领-1（wc-1）和白色领-2（wc-2）基因的产物之间的相互作用建立了分子昼夜时钟装置，称为FRQ-WC-振荡器（FWO），其是产生分子和明显的昼夜节律性所必需的。在携带非功能性frq等位基因的菌株中，无性孢子发育（分生孢子）的昼夜节律在恒定条件下被废除，但分生孢子在温度循环中保持节律。这些温度同步节律的某些特征归因于无FRQ振荡器（FLO）的活动。这种FLO的分子组成尚不清楚。为了测试FLO是否依赖于其他生物钟成分，我们创建了一个在frq，wc-1，wc-2和vivid（vvd）基因中携带缺失的菌株。该ΔFWO菌株中的分生孢子仍然与循环温度程序同步，但温度诱导的节律性与在单个frq敲除菌株中观察到的不同。这些结果和其他证据表明，组件的FWO的温度引起的FLO的一部分。
In Neurospora crassa, the interactions between products of the frequency (frq), frequency-interacting RNA helicase (frh), white collar-1 (wc-1), and white collar-2 (wc-2) genes establish a molecular circadian clockwork, called the FRQ-WC-Oscillator (FWO), which is required for the generation of molecular and overt circadian rhythmicity. In strains carrying nonfunctional frq alleles, circadian rhythms in asexual spore development (conidiation) are abolished in constant conditions, yet conidiation remains rhythmic in temperature cycles. Certain characteristics of these temperature-synchronized rhythms have been attributed to the activity of a FRQ-less oscillator (FLO). The molecular components of this FLO are as yet unknown. To test whether the FLO depends on other circadian clock components, we created a strain that carries deletions in the frq, wc-1, wc-2, and vivid (vvd) genes. Conidiation in this ΔFWO strain was still synchronized to cyclic temperature programs, but temperature-induced rhythmicity was distinct from that seen in single frq knockout strains. These results and other evidence presented indicate that components of the FWO are part of the temperature-induced FLO.