Analysis of Globodera rostochiensis effectors reveals conserved functions of SPRYSEC proteins in suppressing and eliciting plant immune responses.
Analysis of Globodera rostochiensis effectors reveals conserved functions of SPRYSEC proteins in suppressing and eliciting plant immune responses.
复制标题
DOI：
10.3389/fpls.2015.00623
复制
发表时间：
2015
影响因子：
5.6
通讯作者：
Moffett P
中科院分区：
文献类型：
--
作者：
Ali S;Magne M;Chen S;Obradovic N;Jamshaid L;Wang X;Bélair G;Moffett P
马铃薯胞囊线虫(Globodera rostochiensis(Woll.))是马铃薯的重要害虫。植物寄生线虫产生多种效应蛋白，由它们的口针分泌，从而成功地感染它们的宿主。这些蛋白质包括递送到质外体和寄主细胞质的蛋白质。一些罗氏革兰氏菌的效应物已经被鉴定出来，包括几个属于分泌型spry结构域(SPRYSEC)家族的分子。SPRYSEC蛋白是Globodera属植物所特有的，与诱导和抑制寄主防御反应有关。我们通过在本氏烟草和烟草中表达六种不同的罗氏棉SPRYSEC蛋白来测试它们的性质。我们发现所测试的所有SPRYSEC蛋白都能够抑制NB-LRR蛋白诱导的防御反应以及激发子诱导的细胞死亡，这表明防御抑制是该效应蛋白家族成员的共同特征。同时，GrSPRYSEC-15在表达GrSPRYSEC-15的烟草中诱导了对表达GrSPRYSEC-15病毒的抗性反应。这些结果表明，SPRYSEC蛋白可能具有允许它们被植物免疫系统识别的特征。
Potato cyst nematodes (PCNs), including Globodera rostochiensis (Woll.), are important pests of potato. Plant parasitic nematodes produce multiple effector proteins, secreted from their stylets, to successfully infect their hosts. These include proteins delivered to the apoplast and to the host cytoplasm. A number of effectors from G. rostochiensis predicted to be delivered to the host cytoplasm have been identified, including several belonging to the secreted SPRY domain (SPRYSEC) family. SPRYSEC proteins are unique to members of the genus Globodera and have been implicated in both the induction and the repression of host defense responses. We have tested the properties of six different G. rostochiensis SPRYSEC proteins by expressing them in Nicotiana benthamiana and N. tabacum. We have found that all SPRYSEC proteins tested are able to suppress defense responses induced by NB-LRR proteins as well as cell death induced by elicitors, suggesting that defense repression is a common characteristic of members of this effector protein family. At the same time, GrSPRYSEC-15 elicited a defense responses in N. tabacum, which was found to be resistant to a virus expressing GrSPRYSEC-15. These results suggest that SPRYSEC proteins may possess characteristics that allow them to be recognized by the plant immune system.