Photoregulation of RNA digestion by RNase H with azobenzene-tethered DNA
Photoregulation of RNA digestion by RNase H with azobenzene-tethered DNA
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DOI：
10.1021/ja0471976
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发表时间：
2004-09-22
影响因子：
15
通讯作者：
Komiyama, M
中科院分区：
文献类型：
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作者：
Matsunaga, D;Asanuma, H;Komiyama, M
RNaseH通过偶氮苯连接的正义DNA和天然反义DNA的双重作用，有效地光调节了RNA的反义作用。在黑暗中，RNA的消化受到抑制，因为反义DNA与偶氮苯拴系的正义DNA强烈杂交，从而分离出RNA。在紫外光照射下，反义DNA通过反式-顺式异构化从偶氮苯连接的DNA中释放出来，并与RNA杂交，RNA被RNase H消化。
RNA digestion by RNase H, which is responsible for the antisense effect, was efficiently photoregulated by use of the duplex of azobenzene-tethered sense DNA and native antisense DNA. In the dark, RNA digestion was suppressed because antisense DNA was strongly hybridized with azobenzene-tethered sense DNA, and accordingly RNA was isolated. On UV irradiation, antisense DNA was released from the azobenzene-tethered DNA due to the trans-to-cis isomerization and hybridized with RNA, which was digested by RNase H.