OsCYCP4s coordinate phosphate starvation signaling with cell cycle progression in rice
OsCYCP4s coordinate phosphate starvation signaling with cell cycle progression in rice
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OsCYCP4s 协调磷酸盐饥饿信号与水稻细胞周期进程
DOI：
10.1111/jipb.12885
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发表时间：
2020
影响因子：
11.4
通讯作者：
Yi Keke
中科院分区：
文献类型：
--
作者：
Xu Lei;Wang Fang;Li Ruili;Deng Minjuan;Fu Meilan;Teng Huiying;Yi Keke
磷酸盐饥饿导致作物梢生长和产量的严重下降。茎部生长减少是由缺磷引发的广泛基因表达重编程引起的，而缺磷本身并不是低磷的直接后果。然而，磷酸盐饥饿如何抑制水稻茎部生长尚不清楚。在本研究中，我们确定了oscycp4在水稻对磷酸盐饥饿的响应中调控茎部生长的作用。我们证明了oscycp4的表达水平，除了toscycp4；3、磷酸盐饥饿诱导。磷酸盐饥饿诱导的过表达doscycp4可能与其他细胞周期蛋白竞争，与细胞周期蛋白依赖性激酶结合，从而通过减少细胞增殖来抑制生长。磷酸盐饥饿诱导功能缺失突变体cycp4；1,cycp4；2、andcycp4;4是部分妥协。此外，一些磷酸盐饥饿诱导基因的表达被这些细胞周期蛋白负调控，这表明这些OsCYCP4s也可能参与了磷酸盐饥饿信号传导。我们得出结论，磷酸盐饥饿诱导的OsCYCP4s可能在磷酸盐饥饿胁迫下协调磷酸盐饥饿信号和细胞周期进程。
Phosphate starvation leads to a strong reduction in shoot growth and yield in crops. The reduced shoot growth is caused by extensive gene expression reprogramming triggered by phosphate deficiency, which is not itself a direct consequence of low levels of shoot phosphorus. However, how phosphate starvation inhibits shoot growth in rice is still unclear. In this study, we determined the role ofOsCYCP4sin the regulation of shoot growth in response to phosphate starvation in rice. We demonstrate that the expression levels ofOsCYCP4s, exceptOsCYCP4;3, were induced by phosphate starvation. Overexpression of the phosphate starvation inducedOsCYCP4scould compete with the other cyclins for the binding with cyclin‐dependent kinases, therefore suppressing growth by reducing cell proliferation. The phosphate starvation induced growth inhibition in the loss‐of‐function mutantscycp4;1,cycp4;2, andcycp4;4is partially compromised. Furthermore, the expression of some phosphate starvation inducible genes is negatively modulated by these cyclins, which indicates that these OsCYCP4s may also be involved in phosphate starvation signaling. We conclude that phosphate starvation induced OsCYCP4s might coordinate phosphate starvation signaling and cell cycle progression under phosphate starvation stress.