Fluorescence quenching properties of multiple pyrene-modified RNAs.
Fluorescence quenching properties of multiple pyrene-modified RNAs.
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DOI：
10.1016/j.bmc.2009.05.074
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发表时间：
2009-07
影响因子：
3.5
通讯作者：
Kenji Maie;Mitsunobu Nakamura;T. Takada;K. Yamana
中科院分区：
文献类型：
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作者：
Kenji Maie;Mitsunobu Nakamura;T. Takada;K. Yamana
在2 ' - o糖残基上具有多个芘基甲基取代基的RNA分子可以与互补的RNA序列形成双链，而不会失去热稳定性。在RNA双链中，共价结合的芘可以沿着双链的小凹槽以螺旋方式组装。这些螺旋组装的芘阵列表现出强烈的准分子发射，可以有效地用甲基紫紫素淬灭。
RNA molecules with multiple pyrenylmethyl substituents on the 2′-O-sugar residues can form duplexes with complementary RNA sequences without losing thermal stability. In the RNA duplexes, covalently incorporated pyrenes can assemble in a helical manner along the minor grooves of the duplex. These helically assembled pyrene arrays exhibit intense excimer emissions that are efficiently quenched with methyl viologen.