SHORT UPSTREAM SEQUENCES ASSOCIATED WITH THE MUSCLE-SPECIFIC EXPRESSION OF AN ACTIN GENE IN ASCIDIAN EMBRYOS
SHORT UPSTREAM SEQUENCES ASSOCIATED WITH THE MUSCLE-SPECIFIC EXPRESSION OF AN ACTIN GENE IN ASCIDIAN EMBRYOS
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DOI：
10.1006/dbio.1994.1354
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发表时间：
1994-12-01
影响因子：
2.7
通讯作者：
SATOH, N
中科院分区：
文献类型：
--
作者：
HIKOSAKA, A;KUSAKABE, T;SATOH, N
海鞘（Halocynthia roretzi）胚胎肌动蛋白的HrMA4a基因在分化肌肉细胞的原肠期被激活。HrMA4a转录起始位点附近的5'上游区域包含多个共识序列，包括位于-30的TATA盒，位于-71的e盒，位于-116的CArG盒，以及位于-150和-190之间的三个e盒。当将该区域的缺失构建体与细菌β -半乳糖苷酶（lac-Z）基因融合后，微注射到受精卵中，该报告基因在尾芽胚胎的肌肉细胞中表达。对缺失结构的分析表明，上游103-bp的区域足以满足基因的适当表达。然而，在上游82-bp区域很少检测到P-gal活性，在上游72-bp区域未检测到P-gal活性。近端E-box序列的突变不会干扰报告基因的肌肉特异性表达。这些结果表明，转录起始位点-103和-72核苷酸之间的相当短的序列与HrMA4a的特异性表达有关。(C) 1994学术出版社，Inc.
The HrMA4a gene for an embryonic muscle actin of the ascidian Halocynthia roretzi is activated at the gastrula stage in differentiating muscle cells exclusively. The 5' upstream region close to the transcription start site of HrMA4a contains several consensus sequences, which include a TATA box at -30, and E-box at -71, a CArG box at -116, and a cluster of three E-boxes between -150 and -190. When deletion constructs of this region, fused with the bacterial gene for beta-galactosidase (lac-Z), were microinjected into fertilized eggs, the reporter gene was expressed in muscle cells of tailbud embryos. Analyses of the deletion constructs suggested that the 103-bp upstream region is sufficient for the appropriate expression of the gene. However, P-gal activity was very rarely detectable in the case of 82-bp upstream region and no activity was detected in the case of 72-bp upstream region. Mutations in the proximal E-box sequence did not disturb the muscle-specific expression of the reporter gene. These results suggest that rather short sequences between nucleotides -103 and -72 from the transcription start site are associated with the specific expression of HrMA4a. (C) 1994 Academic Press, Inc.