Glomalin-related soil protein contains non-mycorrhizal-related heat-stable proteins, lipids and humic materials
Glomalin-related soil protein contains non-mycorrhizal-related heat-stable proteins, lipids and humic materials
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DOI：
10.1016/j.soilbio.2010.12.010
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发表时间：
2011-04-01
影响因子：
9.7
通讯作者：
Blyth, Robert I. R.
中科院分区：
文献类型：
--
作者：
Gillespie, Adam W.;Farrell, Richard E.;Blyth, Robert I. R.
据报道，球囊霉素是一种稳定且持久的蛋白质，由菌根真菌大量产生，可能是土壤中有机氮的重要来源。然而，球囊霉素相关的土壤蛋白（GRSP），只有操作定义的提取方法，并已在最好的只有很少的特点。本研究的目的是表征GRSR内的分子结构，基于同步加速器的X射线吸收近边结构（XANES）光谱和热解场电离质谱（Py-FIMS）显示，GRSP包含蛋白质的财团沿着许多杂质。采用蛋白质组学技术，我们发现球囊霉素本身可能是一个硫氧还蛋白的伴侣，然而，没有同源性与蛋白质或DNA的菌根起源进行检测。蛋白质组学技术进一步揭示，这部分含有大量的土壤相关的热稳定蛋白质和非菌根来源的蛋白质。这项研究的结果表明，目前的提取程序，定义GRSP产生的化合物的混合物，从而高估了球囊霉素股票时，使用布拉德福德测定定量。球囊霉素的化学性质尚未最终确定;不可能从提取为GRSR的混合物中阐明球囊霉素的化学结构。相反，对目前用于定义GRSP的免疫反应性测定的特定生物化学的研究，其次是单菌菌根培养物的蛋白质组学特征可能需要推进我们对GRSP的化学性质和农学意义的理解在土壤中。(C)2010爱思唯尔有限公司版权所有。
Glomalin is reportedly a stable and persistent protein produced in copious quantities by mycorrhizal fungi and may be an important pool of organic N in soil. Glomalin-related soil protein (GRSP), however, is only operationally defined by its extraction method, and has been only poorly characterized at best. The goal of this study was to characterize the molecular structures within GRSR Synchrotron-based X-ray absorption near-edge structure (XANES) spectroscopy and pyrolysis field-ionization mass spectrometry (Py-FIMS) revealed that GRSP contains a consortium of proteins along with many impurities. Employing proteomic techniques, we found that glomalin itself may be a thioredoxin-containing chaperone; however, no homologies with proteins or DNA of mycorrhizal origin were detected. Proteomics techniques further revealed that this fraction contains large amounts of soil-related heat-stable proteins and proteins of non-mycorrhizal origin. Results of this research show that the current extraction procedure that defines GRSP yields a mixture of compounds and thereby overestimates glomalin stocks when quantified using the Bradford assay. The chemical nature of glomalin has yet to be conclusively determined; it is unlikely that the chemical structure of glomalin can be elucidated from the mixture extracted as GRSR Instead, an investigation into the specific biochemistry of immunoreactive assays currently used to define GRSP, followed by proteomic characterization of monoxenic mycorrhizal cultures may be required to advance our understanding of the chemical nature and agronomic significance of GRSP in soils. (C) 2010 Elsevier Ltd. All rights reserved.