Alterations of the dominant faecal bacterial groups in patients with Crohn's disease of the colon
Alterations of the dominant faecal bacterial groups in patients with Crohn's disease of the colon
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作者：
Seksik, P;Rigottier-Gois, L;Doré, J
背景和目标：克罗恩病（Crohn's disease，CD）的发病机制与结肠菌群有关，但只有不到30%的菌群是可培养的。我们使用非培养技术研究了缓解期结肠CD患者（n = 9）、活动期结肠CD患者（n = 8）和健康志愿者（n = 16）之间粪便微生物群的潜在差异。用6种放射性标记的16S核糖体核糖核酸（rRNA）进行定量斑点杂交靶向寡核苷酸探针用于测量对应于每个系统发生组的rRNA的比例。结果：大肠杆菌在活动期和静止期均明显增加，而在静止期和活动期均无明显变化。与健康对照组（99（7）%）相比，患者（活动期CD和静止期CD分别为65（11）%和69（6）%）的探针加和性显著较低。TTGE配置文件之间的变化显着活跃和静止CD *，但在健康conditions.Conclusion稳定：CD患者的微生物群落的生物多样性仍然很高。肠杆菌在CD中比在健康中更频繁地观察到，并且超过30%的优势植物群属于尚未确定的系统发育组。
Background and aim: The colonic microflora is involved in the pathogenesis of Crohn's disease (CD) but less than 30% of the microflora can be cultured. We investigated potential differences in the faecal microflora between patients with colonic CD in remission (n=9), patients with active colonic CD (n=8), and healthy volunteers (n=16) using culture independent techniques.Methods: Quantitative dot blot hybridisation with six radiolabelled 16S ribosomal ribonucleic acid (rRNA) targeting oligonucleotide probes was used to measure the proportions of rRNA corresponding to each phylogenetic group. Temporal temperature gradient gel electrophoresis (TTGE) of 16S rDNA was used to evaluate dominant species diversity.Results: Enterobacteria were significantly increased in active and quiescent CD. Probe additivity was significantly lower in patients (65 (11)% and 69 (6)% in active CD and quiescent CD) than in healthy controls (99 (7)%). TTGE profiles varied markedly between active and quiescent CD *but were stable in healthy conditions.Conclusion: The biodiversity of the microflora remains high in patients with CD. Enterobacteria were observed significantly more frequently in CD than in health, and more than 30% of the dominant flora belonged to yet undefined phylogenetic groups.