Parvulin (Par14), a Peptidyl-Prolyl cis-trans Isomerase, Is a Novel rRNA Processing Factor That Evolved in the Metazoan Lineage
Parvulin (Par14), a Peptidyl-Prolyl cis-trans Isomerase, Is a Novel rRNA Processing Factor That Evolved in the Metazoan Lineage
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DOI：
10.1074/mcp.m900147-mcp200
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发表时间：
2009-07-01
影响因子：
7
通讯作者：
Takahashi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Fujiyama-Nakamura, Sally;Yoshikawa, Harunori;Takahashi, Nobuhiro
虽然parvulin（Par 14/真核parvulin同系物），肽基脯氨酰顺反异构酶，被发现与前核糖体核糖核蛋白（前rNP）复合物，其在核糖体生物合成的作用仍然没有确定。在这项研究中，我们描述了一个全面的蛋白质组学分析的Par 14相关的前rRNP复合物使用LC-MS/MS和敲除分析Par 14。结合我们以前的结果，我们最终确定了115个蛋白质组分的复合物，包括39个核糖体蛋白和54个潜在的反式作用因子，其酵母同系物中发现的前rRNP复合物形成在核糖体生物合成的各个阶段。我们给出的证据表明，虽然Par 14存在于磷酸化和未磷酸化的形式在细胞中，只有后者的形式与前40 S和前60 S核糖体复合物。我们还表明，Par 14共定位与核仁蛋白B23在间期和纺锤体在有丝分裂和放线菌素D治疗的结果排除Par 14从核仁。最后，我们证明了Par 14 mRNA的敲低使pre-rRNA向18和28 S rRNA的加工减速。我们认为Par 14是前rRNA复合物的一个组成部分，在核糖体生物合成中起rRNA加工因子的作用。由于Par 14的氨基酸序列，包括在氨基末端的前-rRNP结合区是保守的，只有在后生动物同源物，我们建议，它在核糖体生物合成的作用已经演变的后生动物谱系。Molecular & Cellular Proteomics 8：1552-1565，2009.
Although parvulin (Par14/eukaryotic parvulin homolog), a peptidyl-prolyl cis-trans isomerase, is found associated with the preribosomal ribonucleoprotein (pre-rRNP) complexes, its roles in ribosome biogenesis remain undetermined. In this study, we describe a comprehensive proteomics analysis of the Par14-associated pre-rRNP complexes using LC-MS/MS and a knockdown analysis of Par14. Together with our previous results, we finally identified 115 protein components of the complexes, including 39 ribosomal proteins and 54 potential trans-acting factors whose yeast homologs are found in the pre-rRNP complexes formed at various stages of ribosome biogenesis. We give evidence that, although Par14 exists in both the phosphorylated and unphosphorylated forms in the cell, only the latter form is associated with the pre-40 S and pre-60 S ribosomal complexes. We also show that Par14 co-localizes with the nucleolar protein B23 during the interphase and in the spindle apparatus during mitosis and that actinomycin D treatment results in the exclusion of Par14 from the nucleolus. Finally we demonstrate that knockdown of Par14 mRNA decelerates the processing of pre-rRNA to 18 and 28 S rRNAs. We propose that Par14 is a component of the pre-rRNA complexes and functions as an rRNA processing factor in ribosome biogenesis. As the amino acid sequence of Par14 including that in the amino-terminal pre-rRNP binding region is conserved only in metazoan homologs, we suggest that its roles in ribosome biogenesis have evolved in the metazoan lineage. Molecular & Cellular Proteomics 8: 1552-1565, 2009.