Mechanical properties and osteogenic potential of hydroxyapatite-PLGA-collagen biomaterial for bone regeneration
Mechanical properties and osteogenic potential of hydroxyapatite-PLGA-collagen biomaterial for bone regeneration
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DOI：
10.1080/09205063.2016.1184121
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发表时间：
2016-01-01
影响因子：
3.6
通讯作者：
Wick, Timothy M.
中科院分区：
文献类型：
--
作者：
Bhuiyan, Didarul B.;Middleton, John C.;Wick, Timothy M.
骨移植是一种复杂的结构，它提供机械支持和生物信号来调节骨的生长、重建和修复。单一成分的材料不足以提供适当的结构支撑和生物刺激的组合来促进骨再生。多组分复合生物材料缺乏足够的组份之间的结合以防止植入后的相分离。我们已经开发了一种新的多步聚合和制备工艺来构建纳米羟基磷灰石-聚(D，L-丙交酯-共乙交酯)-胶原生物材料(简称nHAP-PLGA-胶原)，并将其各组分共价键合在一起。在本研究中，对nHAP-PLGA-胶原的力学性能和成骨能力进行了表征，以评估该材料是否适合支持骨再生。NHAP-PLGA-胶原膜的抗拉强度与人松质骨的抗拉强度非常接近。人骨髓间充质干细胞(HMSCs)在2DnHAP-PLGA-胶原膜上可存活，培养7d后细胞数量增加7倍。培养5周以上，hMSCs可沉积与成骨分化和骨形成相一致的基质和矿物质。作为基质沉积的结果，含有hMSCs的nHAP-PLGA-胶原膜的拉伸强度比无细胞培养的nHAP-PLGA-胶原膜高48%，弹性系数高5倍。更有趣的是，分化的hMSCs在nHAP-PLGA-胶原膜上培养5周后分泌的基质和矿物质可以减轻PLGA水解所带来的机械强度的损失。
A bone graft is a complicated structure that provides mechanical support and biological signals that regulate bone growth, reconstruction, and repair. A single-component material is inadequate to provide a suitable combination of structural support and biological stimuli to promote bone regeneration. Multicomponent composite biomaterials lack adequate bonding among the components to prevent phase separation after implantation. We have previously developed a novel multistep polymerization and fabrication process to construct a nano-hydroxyapatite-poly(D,L-lactide-co-glycolide)-collagen biomaterial (abbreviated nHAP-PLGA-collagen) with the components covalently bonded to each other. In the present study, the mechanical properties and osteogenic potential of nHAP-PLGA-collagen are characterized to assess the material's suitability to support bone regeneration. nHAP-PLGA-collagen films exhibit tensile strength very close to that of human cancellous bone. Human mesenchymal stem cells (hMSCs) are viable on 2D nHAP-PLGA-collagen films with a sevenfold increase in cell population after 7days of culture. Over 5 weeks of culture, hMSCs deposit matrix and mineral consistent with osteogenic differentiation and bone formation. As a result of matrix deposition, nHAP-PLGA-collagen films cultured with hMSCs exhibit 48% higher tensile strength and fivefold higher moduli compared to nHAP-PLGA-collagen films without cells. More interestingly, secretion of matrix and minerals by differentiated hMSCs cultured on the nHAP-PLGA-collagen films for 5weeks mitigates the loss of mechanical strength that accompanies PLGA hydrolysis.