Altered p-STAT3 (tyr705) expression is associated with histological grading and intratumour microvessel density in hepatocellular carcinoma
Altered p-STAT3 (tyr705) expression is associated with histological grading and intratumour microvessel density in hepatocellular carcinoma
复制标题
DOI：
10.1136/jcp.2006.036970
复制
发表时间：
2007-06-01
影响因子：
3.4
通讯作者：
Lee, King-Teh
中科院分区：
文献类型：
--
作者：
Yang, Sheau-Fang;Wang, Shen-Nien;Lee, King-Teh
背景：酪氨酸残基705 （p-STAT3 (tyr705)）上信号换能器和转录激活子3的组成性激活与许多类型的人类癌症有关。然而，其在肝细胞癌（HCC）中的潜在作用和生物学效应尚未得到很好的证实。目的：探讨p-STAT3 （tyr705）表达的改变是否与血管生成或增殖有关，从而在HCC的发展中起作用。方法：收集69例肝癌患者的石蜡包埋切片。采用半定量免疫组化染色法，分析p-STAT3 （tyr705）在HCC病变及邻近非肿瘤肝实质中的表达规律。结果进一步与肿瘤内微血管密度（MVD）、Ki-67表达、临床病理参数及总生存率相关。结果：49.3%的HCC病变可见强p- stat3 （tyr705）核染色，但仅5.8%的邻近非肿瘤肝实质可见强p- stat3 （tyr705）核染色（p < 0.001）。p- stat3 （tyr705）在HCC病变中的表达与肿瘤内MVD呈显著正相关（p = 0.002），与Ki-67表达无显著正相关。p- stat3 （tyr705）与组织学分级无显著相关性（p = 0.019）。多因素Cox回归分析显示，p- stat3 （tyr705）表达是HCC总生存率的重要预测因子（p = 0.036），尽管Kaplan-Meier生存曲线显示高表达和低表达p- stat3 （tyr705）亚组之间无显著差异。结论：p-STAT3 （tyr705）表达与HCC的组织学分级及肿瘤内MVD密切相关。因此，p-STAT3 （tyr705）在HCC发展中的潜在作用可能是通过这些相关性。
Background: Constitutive activation of signal transducer and activator of transcription 3 at tyrosine residue 705 (p-STAT3 (tyr705)) has been associated with many types of human cancers. However, its potential roles and biological effects in hepatocellular carcinoma (HCC) are not well established.Aim: To explore whether an altered p-STAT3 (tyr705) expression is associated with angiogenesis or proliferation and thereby plays a part in HCC development.Methods: Paraffin-wax-embedded sections from 69 patients with HCC were collected in this study. Using a semiquantitative immunohistochemical staining method, the expression patterns of p-STAT3 (tyr705) in both HCC lesions and the adjacent non-tumorous liver parenchyma were analysed. The results obtained were further correlated with intratumour microvessel density (MVD), Ki-67 expression, clinicopathological parameters and overall survival.Results: A strong p-STAT3 (tyr705) nuclear staining was observed in 49.3% of HCC lesions, but was reported only in 5.8% of the adjacent non-tumorous liver parenchyma (p < 0.001). The expression of p-STAT3 (tyr705) in HCC lesions was significantly and positively correlated with the intratumour MVD (p = 0.002), but not with Ki-67 expression. No significant correlation of p-STAT3 (tyr705) was found in addition to histological grading (p = 0.019). Multivariate Cox regression analysis showed that p-STAT3 (tyr705) expression was a significant predictor of overall survival for HCC (p = 0.036), although the Kaplan-Meier survival curves showed no significant difference between the high and low p-STAT3 (tyr705) expression subgroups.Conclusions: The results showed that p-STAT3 (tyr705) expression was closely correlated with histological grading and intratumour MVD in HCC. Thus, the potential role of p-STAT3 (tyr705) in HCC development may be through these correlations.