An online fiber-optic UV-visible detector for continuous free-flow electrophoresis.
An online fiber-optic UV-visible detector for continuous free-flow electrophoresis.
复制标题
用于连续自由流动电泳的在线光纤紫外可见检测器。
DOI：
10.1002/elps.200500342
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Seliskar,CarlJ
中科院分区：
文献类型：
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作者：
Sanganza,WilsonKM;Ridgway,ThomasH;Stalcup,ApryllM;Seliskar,CarlJ
设计了一种用于连续自由流电泳（CFFE）的PC控制的扫描在线UV检测器，以允许在连接到CFFE装置的48个纵向流动池的一维阵列上进行单个UV波长监测。在检测方案中，UV光顺序地通过每个流动池。该设计集成了在线采集CFFE分离组分的吸收光谱。用苯甲酸标准溶液考察了检测器的性能。使用二磷酸氯喹和4-硝基苯酚作为测试溶质，以确定检测器区分CFFE中分离的分析物的能力。
A PC‐controlled, scanning online UV detector for continuous free‐flow electrophoresis (CFFE) was designed to allow for single UV wavelength monitoring across a 1‐D array of 48 longitudinal flow cells interfaced to a CFFE apparatus. In the detection scheme, the UV light is sequentially passed through each of the flow cells. The design integrates online acquisition of absorbance spectra of components separated by CFFE. Benzoic acid standard solutions were used to examine the performance of the detector. Chloroquine diphosphate and 4‐nitrophenol were used as test solutes to determine the detector's ability to distinguish analytes separated in the CFFE.