Protective role of curcumin in oxidative stress of breast cells
Protective role of curcumin in oxidative stress of breast cells
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DOI：
10.3892/or.2011.1386
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发表时间：
2011-10-01
期刊：
影响因子：
4.2
通讯作者：
Balajee, Adayabalam S.
中科院分区：
文献类型：
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作者：
Calaf, Gloria M.;Echiburu-Chau, Carlos;Balajee, Adayabalam S.
姜黄素(二阿魏酰甲烷)是一种众所周知的抗氧化剂，具有抗增殖和诱导细胞凋亡的作用。用永生化乳腺上皮细胞系MCF-10F建立乳腺癌模型，观察姜黄素的作用。该模型由处于不同转化阶段的人乳腺上皮细胞组成：i)对照细胞系MCF-10F，ii)雌激素处理的细胞系，名为雌激素，iii)恶性细胞系，名为Alpha3，iv)恶性和致癌细胞系，名为Alpha5。姜黄素可减少对照MCF-10F、雌激素和Alpha5细胞中过氧化氢的生成。姜黄素对雌激素细胞株中的核因子kappaB(50 KDa)的表达无明显影响，但可降低其蛋白表达。姜黄素增强MCF-10F和Alpha3细胞中锰超氧化物歧化酶蛋白的表达。结果表明，姜黄素处理的Alpha3和Alpha5细胞株过氧化氢酶蛋白表达增加。姜黄素对MCF-10F细胞的脂质过氧化作用略有降低，但显著(P
Curcumin (diferuloylmethane) is a well known antioxidant that exerts anti-proliferative and apoptotic effects. The effects of curcumin were evaluated in a breast cancer model that was developed with the immortalized breast epithelial cell line, MCF-10F after exposure to low doses of high LET (linear energy transfer) a particles (150 keV/mu m) of radiation, and subsequently cultured in the presence of 17 beta-estradiol (estrogen). This model consisted of human breast epithelial cells in different stages of transformation: i) a control cell line, MCF-10F, ii) an estrogen-treated cell line, named Estrogen, iii) a malignant cell line, named Alpha3 and iv) a malignant and tumorigenic, cell line named Alpha5. Curcumin decreased the formation of hydrogen peroxide in the control MCF-10F, Estrogen and Alpha5 cell lines in comparison to their counterparts. Curcumin had little effect on NF kappa B (50 kDa) but decreased the protein expression in the Estrogen cell line in comparison to their counterparts. Curcumin enhanced manganese superoxide dismutase (MnSOD) protein expression in the MCF-10F and Alpha3 cell lines. Results indicated that catalase protein expression increased in curcumin treated-Alpha3 and Alpha5 cell lines. Curcumin slightly decreased lipid peroxidation in the MCF-10F cell lines, but significantly (P