Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development
Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development
复制标题
宏非编码RNA Meg8和Irm在小鼠胚胎发育中的表达
DOI：
10.1016/j.acthis.2011.07.009
复制
发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Wu, Qiong
中科院分区：
文献类型：
--
作者：
Gu, Tiantian;He, Hongjuan;Wu, Qiong
非编码RNA（ncRNA）Meg 8和Irm是Rian基因的选择性剪接异构体。确定ncRNA的时空表达模式对于理解ncRNA在组织和器官发育过程中的生理作用至关重要。本研究采用原位杂交和实时荧光定量RT-PCR（QRT-PCR）技术，以小鼠胚胎为研究对象，研究了Meg 8和Irm这两种印迹大分子ncRNA的发育调控表达。原位杂交结果显示，Meg 8和Irm在胚胎10.5天和11.5天的脑中均有表达，而Irm在体节中有明显的表达，而Meg 8在体节中没有表达。到E15.5，它们在脑、舌、肝、肺和神经内分泌组织中表达，而Irm在舌和骨骼肌中的表达比Meg 8更受限。此外，定量分析证实它们在E12.5、E15.5和E18.5在舌和脑中高度表达。这些结果表明，Meg 8和Irm可能在不同器官中协同表达，且功能相关。值得注意的是，在胚胎发育过程中，Irm与骨骼肌的形态发生比Meg 8更密切相关。(C)2011年Elsevier GmbH。All rights reserved.
Non-coding RNAs (ncRNAs) Meg8 and Irm were previously identified as alternatively splicing isoforms of Rian gene. Ascertaining ncRNAs spatiotemporal expression patterns is crucial for understanding the physiological roles of ncRNAs during tissue and organ development. In this study in mouse embryos, we focused on the developmental regulation expression of imprinted macro ncRNAs, Meg8 and Irm by using in situ hybridization and quantitative real-time RT-PCR (QRT-PCR). The in situ hybridization results showed that Meg8 and Irm were expressed in the developing brain at embryonic day 10.5 (E10.5) and E11.5, while Irm expression signals were strikingly detected in the somite, where Meg8 expression signals were undetectable. By E15.5, they were expressed in brain, tongue, liver, lung and neuroendocrine tissues, while Irm displayed more restricted expression in tongue and skeletal muscle than Meg8. Furthermore, quantitative analysis confirmed that they were highly expressed in tongue and brain at E12.5, E15.5 and E18.5. These results indicated that Meg8 and Irm might be coordinately expressed and functionally correlated in diverse of organs. Notably, Irm was more closely associated with morphogenesis of skeletal muscle in contrast to Meg8 during embryonic development. (C) 2011 Elsevier GmbH. All rights reserved.