Liquid−Liquid Phase Separation of an Intrinsically Disordered Region of a Germ Cell-Specific Protein Modulates the Stability and Conformational Exchange Rate of SH3 Domain
Liquid−Liquid Phase Separation of an Intrinsically Disordered Region of a Germ Cell-Specific Protein Modulates the Stability and Conformational Exchange Rate of SH3 Domain
复制标题
生殖细胞特异性蛋白质本质无序区域的液相-液相分离调节 SH3 结构域的稳定性和构象交换率
DOI：
10.1021/acs.jpclett.2c01920
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发表时间：
2022
影响因子：
5.7
通讯作者：
Conggang Li
中科院分区：
文献类型：
--
作者：
Liang Luo;Qiong Wu;Shixia Ji;Yixiang Liu;Kai Cheng;Maili Liu;Ling Jiang;Conggang Li
液-液相分离现象存在于许多生物过程中。包裹在相分离液滴中的生物分子经历了与细胞或水溶液中明显不同的环境。在这里，我们定量表征的热力学和交换动力学的模型蛋白SH 3域的凝聚相的一个固有的无序区域的。一个生殖细胞特异性蛋白DDX 4 N1通过使用19 F-NMR光谱。SH 3结构域的and.exchange率与缓冲液和大分子拥挤条件下的稳定率不同。我们的发现表明，局部瞬态有序的微观结构和凝聚物的异质性在调节包膜蛋白的功能中起着重要的作用，这一发现可能是必要的，以进一步了解相分离如何调节细胞中蛋白质的功能。biophysical.properties
The phenomenon of liquid−liquid phase separation is found in numerous.biological processes. The biomolecules enveloped in the phase-separated droplets.experience an obviously different environment from those in cellular or aqueous solution..Herein, we quantitatively characterized the thermodynamics and exchange kinetics of a.model protein SH3 domain in the condensed phase of an intrinsically disordered region of.a germ cell-specific protein DDX4N1 by using 19F-NMR spectroscopy. The stability and.exchange rate of the SH3 domain are different from those in buffer and macromolecular.crowding conditions. Our finding indicates that the local transient ordered microstructure.and heterogeneity in the condensates play significant roles in modulating the biophysical.properties of the enveloped proteins, and this finding may be essential to further our.understanding how phase separation regulates the function of proteins in cells.