DOMAIN-STRUCTURE OF THE SIMIAN VIRUS-40 CORE ORIGIN OF REPLICATION
DOMAIN-STRUCTURE OF THE SIMIAN VIRUS-40 CORE ORIGIN OF REPLICATION
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DOI：
10.1128/mcb.6.5.1663
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发表时间：
1986-05-01
影响因子：
5.3
通讯作者：
TEGTMEYER, P
中科院分区：
文献类型：
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作者：
DEB, S;DELUCIA, AL;TEGTMEYER, P
猿猴病毒40核心复制起点由核苷酸5211至31组成。这64个碱基对包含三个具有严格序列要求的功能结构域和两个具有宽松序列特异性但精确位置限制的间隔区。早期结构域在核苷酸5211和5220之间延伸10个连续碱基对。从序列5221到5229的9个碱基对间隔区将早期结构域与指导T抗原结合的23个碱基对中央回文序列分开。核苷酸12和31之间的核心末端还包含尚未完全定位的间隔区和序列特异性功能。我们提出，序列特异性结构域是病毒和细胞蛋白质的相互作用位点，DNA构象的决定因素，或两者兼而有之。间隔件将这些信号定位在相对于彼此的所需距离和旋转处。
The simian virus 40 core origin of replication consists of nucleotides 5211 through 31. These 64 base pairs contain three functional domains with strict sequence requirements and two spacer regions with relaxed sequence specificity but precise positional constraints. The early domain extends for 10 contiguous base pairs between nucleotides 5211 and 5220. A 9-base pair spacer from sequence 5221 through 5229 separates the early domain from the 23-base pair central palindrome that directs the binding of T antigen. The late end of the core between nucleotides 12 and 31 also contains spacer and sequence-specific functions that are not yet completely mapped. We propose that the sequence-specific domains are interaction sites for viral and cellular proteins, determinants of DNA conformation, or both. The spacers would position these signals at required distances and rotation relative to one another.