Altered Morphologies and Functions of the Olfactory Bulb and Hippocampus Induced by miR-30c.
Altered Morphologies and Functions of the Olfactory Bulb and Hippocampus Induced by miR-30c.
复制标题
miR-30c 诱导嗅球和海马形态和功能的改变
DOI：
10.3389/fnins.2016.00207
复制
发表时间：
2016
影响因子：
4.3
通讯作者：
Ling S
中科院分区：
文献类型：
--
作者：
Sun T;Li T;Davies H;Li W;Yang J;Li S;Ling S
成人的神经发生被认为对大脑有一定程度的可塑性。然而，成年出生的神经元对大脑的影响在很大程度上仍然是未知的。在这里，我们通过立体定向注射各自上调和下调的慢病毒来改变miR-30c在脑室下区(SVZ)和齿状回(DG)的表达。结果显示，miR-30c水平的升高通过促进干细胞的细胞周期来促进成年神经发生，而下调miR-30c的水平则导致相反的结果。当miR-30c的这些作用持续3个月时，我们检测到嗅球(OB)的明显形态变化和海马区的谱系改变。嗅觉敏感性以及联想和空间记忆的测试表明，一定数量的成年出生的神经元对于OB和海马体的正常功能是必不可少的，但也存在多余的新生神经元，这些神经元不会进一步改善这些区域的功能。我们的研究揭示了miRNA、成人神经发生、脑形态和功能之间的相互作用，这为理解新生神经元在成人大脑中的作用提供了新的见解。
Adult neurogenesis is considered to contribute to a certain degree of plasticity for the brain. However, the effects of adult-born neurons on the brain are still largely unknown. Here, we specifically altered the expression of miR-30c in the subventricular zone (SVZ) and dentate gyrus (DG) by stereotaxic injection with their respective up- and down-regulated lentiviruses. Results showed an increased level of miR-30c enhanced adult neurogenesis by prompting cell-cycles of stem cells, whereas down-regulated miR-30c led to the opposite results. When these effects of miR-30c lasted for 3 months, we detected significant morphological changes in the olfactory bulb (OB) and lineage alteration in the hippocampus. Tests of olfactory sensitivity and associative and spatial memory showed that a certain amount of adult-born neurons are essential for the normal functions of the OB and hippocampus, but there also exist redundant newborn neurons that do not further improve the functioning of these areas. Our study revealed the interactions between miRNA, adult neurogenesis, brain morphology and function, and this provides a novel insight into understanding the role of newborn neurons in the adult brain.
影响因子：
4.3
作者：
De Marchis S;Puche AC
通讯作者：
Puche AC