Comparison between two rabbit models of posttraumatic osteoarthritis: A longitudinal tear in the medial meniscus and anterior cruciate ligament transection.
Comparison between two rabbit models of posttraumatic osteoarthritis: A longitudinal tear in the medial meniscus and anterior cruciate ligament transection.
复制标题
两种兔创伤后骨关节炎模型的比较：内侧半月板纵向撕裂和前十字韧带横断。
DOI：
10.1002/jor.24645
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发表时间：
2020
影响因子：
2.8
通讯作者：
Wu Li Dong
中科院分区：
文献类型：
--
作者：
Huang Kai;Cai Hai Li;Zhang Peng Li;Wu Li Dong
已经开发了动物骨关节炎（OA）模型以了解OA进展和评估新的OA疗法。然而，关节病变的个体差异仍然是当前大多数OA模型中的关键问题。我们建立了一种新的兔模型，通过创建一个纵向撕裂内侧半月板体，是可重复的和类似的创伤后生物力学紊乱在人类OA。新西兰兔接受手术并评估9周。将家兔随机分为假手术对照组、内侧膝关节撕裂（MMT）组和前交叉韧带横断（ACLT）组。在给药后4、6和9周处死动物。收集膝关节进行组织学和基因表达评估。MMT和ACLT手术均导致股骨髁软骨发生时间依赖性退行性变化。在每个时间点，MMT组软骨显示出比ACLT组软骨更严重的退行性变化。结论：MMT组炎性细胞因子和分解代谢基因表达明显高于ACLT组，而合成代谢基因表达明显低于ACLT组。MMT治疗引起更严重的结构损伤的软骨和更高的分解代谢基因表达水平比ACLT模型在每个时间点。MMT模型在研究创伤后OA（PTOA）的发展，特别是骨关节损伤的PTOA方面可能非常有益。MMT模型复制了人类PTOA的关键特征，包括关节病变、炎症反应和骨关节炎软骨退化的进展，从而为将有希望的治疗转化为临床实践提供了一条令人兴奋的新途径。
Animal osteoarthritis (OA) models have been developed to understand OA progression and evaluate new OA therapies. However, individual variations in joint lesions remain a critical problem in most current OA models. We established a novel rabbit model by creating a longitudinal tear in the medial meniscus body that was reproducible and similar to posttraumatic biomechanical disturbances in human OA. New Zealand rabbits underwent surgery and were assessed for 9 weeks. The rabbits were randomized into the sham control, medial meniscal tear (MMT), and anterior cruciate ligament transection (ACLT) groups. The animals were sacrificed at 4, 6, and 9 weeks posttreatment. The knee joints were harvested for histological and gene expression assessments. Both the MMT and ACLT procedures led to time‐dependent degenerative changes in the femoral condyle cartilage. At each time point, the MMT group cartilage showed more severe degenerative changes than did the ACLT group cartilage. Consistently, inflammatory cytokine and catabolic gene expression were significantly higher, and anabolic gene expression was significantly lower in the MMT group than in the ACLT group. MMT treatment caused more severe structural damage to the cartilage and higher catabolic gene expression levels than the ACLT model at each time point. The MMT model may be highly beneficial in investigating posttraumatic OA (PTOA) development, especially PTOA from a meniscal injury. The MMT model replicated key features of human PTOA, including meniscal lesions, inflammatory responses, and the progression to osteoarthritic cartilage degeneration, thereby providing an exciting new avenue for translating promising treatments to clinical practice.