Clinicopathological characteristics of Xp11.2 translocation renal cell carcinoma in adolescents and adults: Diagnosis using immunostaining of transcription factor E3 and fluorescence in situ hybridization analysis
Clinicopathological characteristics of Xp11.2 translocation renal cell carcinoma in adolescents and adults: Diagnosis using immunostaining of transcription factor E3 and fluorescence in situ hybridization analysis
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DOI：
10.1111/iju.13007
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发表时间：
2016-02-01
影响因子：
2.6
通讯作者：
Tsukamoto, Taiji
中科院分区：
文献类型：
--
作者：
Hirobe, Megumi;Masumori, Naoya;Tsukamoto, Taiji
目的通过转录因子E3免疫染色和荧光原位杂交分析确定Xp11.2易位癌的发生率和临床病理特征。方法我们通过检查所有苏木精-伊红染色切片并对所有病例进行转录因子E3免疫染色，对1990年至2009年在日本札幌医科大学医院治疗的638例肾细胞癌患者进行评估。对免疫染色阳性或苏木精-伊红染色切片上疑似 Xp11.2 易位癌的患者进行荧光原位杂交分析。在此分析中，我们设置了至少 10% 细胞核的分裂信号的截止水平。结果 在 631 名患者中，20 名 (3.2%) 免疫染色呈阳性。最终，5 名患者被诊断为 Xp11.2 易位癌（0.8%）。其中4例为女性，年龄小于50岁，3例诊断为IV期，有多发区域淋巴结或内脏转移。免疫染色的阳性预测值为25%。结论Xp11易位肾细胞癌患者往往较年轻、女性多见且诊断时处于晚期。免疫染色后进行荧光原位杂交分析是诊断 Xp11 易位肾细胞癌的一种准确且经济有效的方法。
ObjectivesTo determine the rate and clinicopathological features of Xp11.2 translocation carcinoma using immunostaining of transcription factor E3 and fluorescence in situ hybridization analysis.MethodsWe evaluated 638 patients with renal cell carcinoma treated at Sapporo Medical University Hospital, Sapporo, Japan, from 1990 to 2009 by reviewing all hematoxylin-eosin-stained sections and carrying out immunostaining of transcription factor E3 for all cases. Fluorescence in situ hybridization analysis was carried out for patients with positive immunostaining or with findings suspicious for Xp11.2 translocation carcinoma on hematoxylin-eosin-stained sections. In this analysis, we set a cut-off level for split signals of at least 10% of nuclei.ResultsOf the 631 patients, 20 (3.2%) were positive for immunostaining. Finally, five patients were diagnosed with Xp11.2 translocation carcinoma (0.8%). Four of these patients were female and aged less than 50 years, and three cases were diagnosed as stage IV with multiple regional lymph nodal or visceral metastases. The positive predictive value of immunostaining was 25%.ConclusionPatients with Xp11 translocation renal cell carcinoma tend to be younger, more frequently female and diagnosed at a more advanced stage. Immunostaining followed by fluorescence in situ hybridization analysis is an accurate and cost-effective approach for diagnosis of Xp11 translocation renal cell carcinoma.