FUS3 REPRESSES CLN1 AND CLN2 AND IN CONCERT WITH KSS1 PROMOTES SIGNAL TRANSDUCTION
FUS3 REPRESSES CLN1 AND CLN2 AND IN CONCERT WITH KSS1 PROMOTES SIGNAL TRANSDUCTION
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DOI：
10.1073/pnas.88.21.9392
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发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
FINK, GR
中科院分区：
文献类型：
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作者：
ELION, EA;BRILL, JA;FINK, GR
FUS 3在鸟嘌呤核苷酸结合蛋白（G蛋白）的β-亚基STE 4和交配型特异性转录激活因子STE 12之间的信号转导步骤中与同源酵母蛋白激酶KSS 1功能冗余。FUS 3或KSS 1都可以执行此功能;当编码这些蛋白激酶的基因都不存在时，信号转导被阻断，导致不育。这种功能冗余是菌株依赖性的;一些标准实验室菌株（S288 C）是kss 1-。FUS 3具有细胞周期停滞和营养生长所需的额外功能，这些功能与KSS 1功能不重叠。FUS 3通过转录抑制两个G1期细胞周期蛋白（CLN 1和CLN 2）和通过转录后抑制第三个G1期细胞周期蛋白（CLN 3）介导细胞周期停滞。FUS 3也需要在单倍体菌株的营养生长依赖于CLN 3的细胞周期进程，但不需要在依赖于CLN 1或CLN 2的菌株，这表明三个G1细胞周期蛋白之间的功能分歧。FUS 3的不同作用表明FUS 3蛋白激酶具有多种底物，其中一些可能与KSS 1共享。
FUS3 is functionally redundant with KSS1, a homologous yeast protein kinase, for a step(s) in signal transduction between the beta-subunit of the guanine nucleotide binding protein (G protein), STE4, and the mating type-specific transcriptional activator, STE12. Either FUS3 or KSS1 can execute this function; when neither gene encoding these protein kinases is present, signal transduction is blocked, causing sterility. This functional redundancy is strain dependent; some standard laboratory strains (S288C) are kss1-. FUS3 has additional functions required for cell cycle arrest and vegetative growth that do not overlap with KSS1 functions. FUS3 mediates cell cycle arrest during mating through transcriptional repression of two G1 cyclins (CLN1 and CLN2) and through posttranscriptional inhibition of a third G1 cyclin (CLN3). FUS3 is also required for vegetative growth in haploid strains dependent upon CLN3 for cell cycle progression but is not required in strains dependent upon either CLN1 or CLN2, suggesting a functional divergence among the three G1 cyclins. The diverse roles for FUS3 suggest that the FUS3 protein kinase has multiple substrates, some of which may be shared with KSS1.