Effects of repetitive and short time strain in human bone marrow stromal cells
Effects of repetitive and short time strain in human bone marrow stromal cells
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DOI：
10.1002/jbm.a.31944
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发表时间：
2009-03-15
影响因子：
4.9
通讯作者：
van Griensven, Martijn
中科院分区：
文献类型：
--
作者：
Diederichs, Solvig;Freiberger, Friedrich;van Griensven, Martijn
为了长期的目标，工程的功能性骨结构，我们施加单向循环机械应变的人骨髓基质细胞。施加两种应变制度，分别包括单一和重复应变。对于单一应变，我们施加15和60分钟的机械载荷（1 Hz，5%延伸率）。15分钟和60分钟负荷后检测到增殖和I型胶原水平增加，同时60分钟应变后III型胶原产量增加。为了研究可能的效应放大，连续3天施加长时间应变，每天8小时应变持续时间，以诱导持续和明显的细胞反应。此外，观察到钙化增加。所观察到的变化与p38、细胞外信号调节激酶或c-jun N-末端激酶活化（Western Blot）的变化无关。总之，应用长达60分钟的单菌株不足以诱导持续的细胞反应。15分钟似乎诱导有益的效果，而60分钟的应变诱导III型胶原蛋白（作为疤痕形成的标志）。重复菌株（3 × 8小时）诱导增强的分化，而没有有害的副作用。(c)2008 Wiley Periodicals，Inc. J Biomed Mater Res 88A：907-915，2009
For the long-term objective to engineer a functional bone construct, we applied unidirectional cyclic mechanical strain to human bone marrow stromal cells. Two strain regimes were applied comprising single and repetitive strain, respectively. For the single strain, we applied 15 and 60 min of mechanical load (1 Hz, 5% elongation). Increased proliferation and type I collagen levels were detected after 15- and 60-min load accompanied by increased type III collagen production after 60 min of strain. To study a possible amplification of effects, long time strain on 3 consecutive days, with every day 8 h strain duration, was applied in order to induce persistent and evident cellular reactions. Furthermore, an increased calcification was observed. The observed changes were not associated with changes in p38, extracellular signal regulated kinase or c-jun N-terminal kinase activation (Western Blot). In conclusion, application of a single strain period of up to 60 min is not sufficient to induce persistent cellular reactions. Fifteen minutes seems to induce beneficial effects, whereas 60 min of strain induces collagen type III (as a sign of scar formation). Repetition of strain (3 X 8 h) induced enhanced differentiation without detrimental side effects. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 88A: 907-915, 2009