Microarray analysis of brassinosteroid-regulated genes in Arabidopsis
Microarray analysis of brassinosteroid-regulated genes in Arabidopsis
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DOI：
10.1104/pp.011254
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发表时间：
2002-11-01
期刊：
影响因子：
7.4
通讯作者：
Yoshida, S
中科院分区：
文献类型：
--
作者：
Goda, H;Shimada, Y;Yoshida, S
油菜素内酯（BRs）是类固醇植物激素，对植物的生长和发育至关重要。尽管近期对生物合成和敏感性突变体的研究已对BRs的功能有所了解，但对其作用方式却知之甚少。通过使用DNA微阵列分析，我们在拟南芥野生型（WT；哥伦比亚生态型）以及BR缺乏型突变体det2中鉴定了受BR调控的基因。受BR调控的基因在det2突变体中的反应通常比在野生型中更强烈，而在对BR不敏感的突变体bri1中仅表现出有限的反应。一小部分基因在野生型中的反应比在det2中更强。用油菜素内酯和油菜素唑（一种BR生物合成的特异性抑制剂）处理植物，对所鉴定基因的表达产生了相反的影响。受BR调控的基因列表包括转录因子基因，如光敏色素相互作用因子3、生长素相关基因、P450基因以及与细胞伸长和细胞壁组织有关的基因。此处呈现的结果以受BR调控的基因作为分子标记，提供了对BRs生理功能的全面了解。受BR调控的基因列表将有助于对与BR功能相关的新突变体和新的生长调节化合物进行表征。
Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. Although insights into the functions of BRs have been provided by recent studies of biosynthesis and sensitivity mutants, the mode of action of BRs is poorly understood. With the use of DNA microarray analysis, we identified BR-regulated genes in the wild type (WT; Columbia) of Arabidopsis and in the BR-deficient mutant, det2. BR-regulated genes generally responded more potently in the det2 mutant than in the WT, and they showed only limited response in a BR-insensitive mutant, bri1. A small group of genes showed stronger responses in the WT than in the det2. Exposure of plants to brassinolide and brassinazole, which is a specific inhibitor of BR biosynthesis, elicited opposite effects on gene expression of the identified genes. The list of BR-regulated genes is constituted of transcription factor genes including the phytochrome-interacting factor 3, auxin-related genes, P450 genes, and genes implicated in cell elongation and cell wall organization. The results presented here provide comprehensive view of the physiological functions of BRs using BR-regulated genes as molecular markers. The list of BR-regulated genes will be useful in the characterization of new mutants and new growth-regulating compounds that are associated with BR function.