Expression of peptide YY in human brain and pituitary tissues
Expression of peptide YY in human brain and pituitary tissues
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DOI：
10.1016/j.nut.2008.06.011
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发表时间：
2008-09-01
期刊：
影响因子：
4.4
通讯作者：
Takahashi, Kazuhiro
中科院分区：
文献类型：
--
作者：
Morimoto, Ryo;Satoh, Fumitoshi;Takahashi, Kazuhiro
应用放射免疫分析、免疫细胞化学和逆转录聚合酶链反应研究了人脑和垂体组织中肽YY（PYY）的表达。用兔抗人PYY 1 -36的多克隆抗体与PYY 3 -36的交叉反应率为100%，与神经肽Y和胰多肽等其他多肽无明显交叉反应。免疫反应性PYY的最高浓度见于下丘脑（0.44 +/- 0.060 pmol/g湿重，平均值+/- SEM，n = 8），其次是垂体（0.41 +/- 0.16 pmol/g湿重，n = 3）。反相高效液相色谱法的组织提取物的人直肠和皮质脑显示出一个峰洗脱的位置正宗PYY 1 -36和PYY 3 -36。免疫细胞化学显示，阳性免疫染色PYY的室旁核，弓状核，视上核的人下丘脑的神经元。此外，逆转录聚合酶链反应分析表明，在人脑和垂体组织中表达PYY的mRNA。本研究首次显示PYY在人脑和垂体组织中表达，表明PYY作为神经递质在神经内分泌生理学中的作用，例如调节食欲和能量消耗以及调节垂体激素分泌。(C)2008年爱思唯尔公司All rights reserved.
Expression of peptide YY (PYY) in the human brain and pituitary tissues was studied by radioimmunoassay, immunocytochemistry, and reverse transcription polymerase chain reaction. The polyclonal antibody raised against human PYY1-36 in a rabbit was used in the assay, which showed 100% cross-reactivity with PYY3-36 and no significant cross-reactivity with other peptides including neuropeptide Y and pancreatic polypeptide. The highest concentration of immunoreactive PYY was found in the hypothalamus (0.44 +/- 0.060 pmol/g of wet weight, mean +/- SEM, n = 8), followed by the pituitary (0.41 +/- 0.16 pmol/g of wet weight, n = 3). Reverse-phase high performance liquid chromatography of tissue extracts of human rectum and cortical brain showed a peak eluted in the position of authentic PYY1-36 and PYY3-36. Immunocytochemistry showed positive immunostaining for PYY in neurons of the paraventricular, arcuate, and supraoptic nuclei of the human hypothalamus. Moreover, reverse transcription polymerase chain reaction analysis showed expression of mRNA for PYY in human brain and pituitary tissues. The present study has shown for the first time expression of PYY in the human brain and pituitary tissues, suggesting a role for PYY as a neurotransmitter, in the neuroendocrine physiology, such as regulation of appetite and energy expenditure and modulation of pituitary hormone secretion. (C) 2008 Elsevier Inc. All rights reserved.