Dual-Functionality Evaluation of a Novel Collagen Crosslinking Resin.
Dual-Functionality Evaluation of a Novel Collagen Crosslinking Resin.
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新型胶原交联树脂的双功能评估。
DOI：
10.1177/00220345211007428
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发表时间：
2021
影响因子：
7.6
通讯作者：
Li,S
中科院分区：
文献类型：
--
作者：
Wang,Y;Liu,Y;Liu,H;Li,S
目前的粘合剂通过混合层内的微联锁机制与牙本质结合。除了这种机械保持外，与牙本质的结合还受益于胶原蛋白和树脂之间的额外化学相互作用。本研究旨在合成一种新型的光固化胶原交联剂甲基丙烯酸酯（MA）功能化葡萄籽提取物（GSE），并评估MAGSE在临床相关环境下交联牙本质胶原的能力，以及它作为树脂在光固化中的作用。通过GSE与甲基丙烯酰氯反应得到MAGSE，实现了MA的功能化，并通过1h - nmr和傅里叶变换红外光谱（FTIR）对其进行了表征。从第三磨牙牙本质板上取下6µm厚的牙本质膜。脱矿后，用1% MAGSE治疗30 s。通过FTIR、基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF）和平板扫描电镜(SEM)/透射电镜（TEM）对胶原交联和MAGSE消化抗性进行评价。同时，将1% MAGSE或GSE加入到由2-甲基丙烯酸羟乙酯和三组分光引发剂组成的实验粘合剂中。用ftir衰减全反射连续实时监测聚合动力学10 min。结果表明，MAGSE对牙本质胶原具有与GSE同样强的结合和抗胶原酶降解作用。1% MAGSE处理30 s的牙本质胶原蛋白在0.1%胶原酶中处理1 h后几乎未被消化（1.6±1.6%），而未处理的胶原蛋白完全被消化（100.9±20.2%）。SEM/TEM图像显示，MAGSE在30秒内有效地交联了牙本质胶原，并使其在临床相关设置下几乎无法消化。与GSE阻碍HEMA光固化不同，MAGSE加速了聚合速度，并表现出具有多个可聚合单元的树脂单体的典型特征。综上所述，合成了一种新型胶原交联树脂MAGSE，它继承了GSE的胶原交联能力和MA的聚合功能。将这种可光固化的胶原交联剂加入到粘接剂中可能是一种革命性的方法，可以提高复合修复体中牙本质结合的耐久性。
Current adhesives bond to dentin via a micro-interlocking mechanism within the hybrid layer. Besides such mechanical retention, bonding to dentin would benefit from additional chemical interaction between collagen and resin. This study aims to synthesize a novel light-curable collagen crosslinker methacrylate (MA) functionalized grapeseed extract (GSE) and to assess MAGSE’s ability to crosslink dentin collagen in a clinically relevant setting as well as its role in light-cure as a resin. MA functionalization was accomplished by reacting GSE with methacryloyl chloride to obtain MAGSE, which was characterized by1H-NMR and Fourier transformed infrared spectroscopy (FTIR). The 6-µm-thick dentin films were microtomed from dentin slabs of third molars. Following demineralization, they were treated for 30 s by 1% MAGSE. Collagen crosslinking and resistance to digestion of MAGSE were evaluated by FTIR, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) assay of films, and scanning electron microscopy (SEM)/transmission electron microscopy (TEM) on slabs. Meanwhile, 1% MAGSE or GSE was added to an experimental adhesive formulated with 2-hydroxyethyl methacrylate and a tricomponent photoinitiator system. Polymerization kinetics were monitored continuously in real time for 10 min using FTIR–attenuated total reflection. The results indicated that MAGSE could bind to dentin collagen and protect it from collagenase degradation as strong as GSE. Dentin collagen treated by 1% MAGSE for 30 s was scarcely digested (1.6 ± 1.6%) after 1 h in 0.1% collagenase, while untreated collagen was completely digested (100.9 ± 20.2%). SEM/TEM images indicated MAGSE efficiently crosslinked dentin collagen in 30 s and rendered it almost inert to digestion under clinically relevant settings. Unlike GSE that hindered light-curing of HEMA, MAGSE accelerated the rate of polymerization and exhibited typical traits of a resin monomer with multiple polymerizable units. In conclusion, a novel collagen crosslinking resin MAGSE is synthesized, which inherits collagen crosslinking ability from GSE and polymerization function from MA. Inclusion of this light-curable collagen crosslinker into adhesives might be a revolutionary way to improve durability of dentin bonding in composite restorations.